Sasaki K, Takeda M, Uchiyama M
J Assoc Off Anal Chem. 1976 Sep;59(5):1118-21.
A method is presented for the gas-liquid chromatographic determination of chloramphenicol in agricultural crops. Chloramphenicol is extracted with ethyl acetate, cleaned up by silicic acid column chromatography, acetylated with acetic anhydride and pyridine, and then measured by gas-liquid chromatography with electron capture detection. Two stationary phases, DEGS + phosphoric acid and Reoplex 400, were used. The sensitivity was ca 8 ng (40% full scale deflection). The efficicency of the analytical method was evaluated by analyzing crops fortified with chloramphenicol. The average recovery ranged from 72% in unpolished rice to 86% in Chinese radishes.
介绍了一种用于气相色谱法测定农作物中氯霉素的方法。氯霉素用乙酸乙酯萃取，通过硅酸柱色谱净化，用乙酸酐和吡啶乙酰化，然后用带有电子捕获检测的气相色谱法进行测定。使用了两种固定相，即DEGS + 磷酸和Reoplex 400。灵敏度约为8 ng（满量程偏转的40%）。通过分析添加了氯霉素的农作物来评估该分析方法的效率。平均回收率范围从未抛光大米中的72%到白萝卜中的86%。
