Luppi M, Longo G, Ferrari M G, Barozzi P, Marasca R, Morselli M, Valenti C, Mascia T, Vandelli L, Vallisa D, Cavanna L, Torelli G
Department of Medical Sciences, University of Modena, Italy.
Ann Oncol. 1998 May;9(5):495-8. doi: 10.1023/a:1008255830453.
Epidemiological evidence has suggested an association between hepatitis C virus (HCV) infection and B-cell lymphoproliferation. We studied the prevalence of HCV infection in a series of de novo B-cell non-Hodgkin's lymphoma (B-NHL) cases and correlated virological findings with clinico-histological features.
One hundred fifty-seven patients with de novo B-NHL were included in the study. Their serum was examined by ELISA and RIBA for the presence of anti-HCV antibodies, and either the peripheral blood mononuclear cells or the pathology tissues of all of the patients were examined by reverse transcriptase polymerase chain reaction for the presence of HCV RNA sequences.
HCV infection occurred in 22.3% of B-NHL patients and was documented before the diagnosis in about half of the positive cases. Of interest, HCV infection was more frequently found in follicular center, marginal zone and diffuse large-cell lymphoma types, but was not associated with symptomatic cryoglobulinemia. The median survival time was 48 months in HCV-positive and 52 months in HCV-negative B-NHL patients.
Our findings strengthen the pathogenetic link between HCV and B-NHL and show that HCV infection may be associated with the malignant proliferation of defined B-cell subsets other than the immunoglobulin Mk B-cell subset involved in the pathogenesis of mixed cryoglobulinemia type II and associated lymphoplasmacytoid lymphoma type. HCV-related liver disease did not affect the survival of our B-NHL patients.
流行病学证据表明丙型肝炎病毒（HCV）感染与B细胞淋巴增殖之间存在关联。我们研究了一系列初发B细胞非霍奇金淋巴瘤（B-NHL）病例中HCV感染的患病率，并将病毒学结果与临床组织学特征相关联。
157例初发B-NHL患者纳入本研究。通过酶联免疫吸附测定（ELISA）和重组免疫印迹法（RIBA）检测他们血清中抗HCV抗体的存在，并且通过逆转录聚合酶链反应检测所有患者的外周血单个核细胞或病理组织中HCV RNA序列的存在。
22.3%的B-NHL患者发生HCV感染，约一半阳性病例在诊断前就有记录。有趣的是，HCV感染在滤泡中心型、边缘区型和弥漫大细胞淋巴瘤类型中更常见，但与有症状的冷球蛋白血症无关。HCV阳性的B-NHL患者中位生存时间为48个月，HCV阴性的为52个月。
我们的研究结果强化了HCV与B-NHL之间的致病联系，并表明HCV感染可能与特定B细胞亚群的恶性增殖有关，而不是与II型混合性冷球蛋白血症及相关淋巴浆细胞样淋巴瘤发病机制中涉及的免疫球蛋白Mκ B细胞亚群有关。HCV相关肝病并未影响我们B-NHL患者的生存。
