Chenevier P, Massias L, Gueylard D, Farinotti R
Service de Pharmacie Clinique et des Biomatériaux, Hopital Bichat, Paris, France.
J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):310-5. doi: 10.1016/s0378-4347(97)00641-5.
A new HPLC assay using UV detection (200 nm) was developed to determine ethambutol (EMB) concentrations in plasma. Following extraction (0.1 ml plasma) with chloroform, EMB and octylamine (used as internal standard) were derivatized with phenylethylisocyanate. Quantitation in plasma was achieved at 200 nm. There were no interferences from endogenous compounds. Intra- and inter-day variabilities were lower than 5.2 and 7.6%, respectively. The limit of quantitation of the method was 0.2 microg/ml. In plasma, ethambutol was found to be stable for at least one month when samples were stored at -20 degrees C. This assay was applied to the therapeutic monitoring of EMB concentrations in 19 patients suffering from tuberculosis.
开发了一种使用紫外检测（200nm）的高效液相色谱法来测定血浆中乙胺丁醇（EMB）的浓度。用氯仿萃取（0.1ml血浆）后，EMB和辛胺（用作内标）用苯乙基异氰酸酯进行衍生化。在200nm处实现血浆中的定量。没有内源性化合物的干扰。日内和日间变异性分别低于5.2%和7.6%。该方法的定量限为0.2μg/ml。在血浆中，当样品储存在-20℃时，发现乙胺丁醇至少稳定一个月。该测定法应用于19例结核病患者EMB浓度的治疗监测。
