微小隐孢子虫与艾美耳属原虫之间免疫交叉反应性的测定

Determination of immuno-cross-reactivity between Cryptosporidium parvum and Eimeria spp.

作者信息

Lorenzo M J, Casal J A, Freire F, Castro J A, Vergara C A, Ares-Mazás M E

机构信息

Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, La Coruña, Spain.

出版信息

Vet Parasitol. 1998 Mar 31;76(1-2):1-8. doi: 10.1016/s0304-4017(97)00225-2.

DOI:10.1016/s0304-4017(97)00225-2

PMID:9653985

Abstract

Immuno-cross-reactivity between Cryptosporidium parvum and Eimeria spp. was studied by the indirect fluorescent antibody test (IFAT) and Western blot procedure. Thirty-seven sera from asymptomatic (non-diarrheic) cattle, with known coprological (presence-absence of coccidia) and serological data respecting C. parvum, were tested by IFAT using Eimeria oocysts as antigen. Most sera (54%) displayed immunofluorescence around the surface of the Eimeria oocysts. Simultaneously, serum samples from rabbits naturally infected with Eimeria spp. (E. magna, E. intestinalis and E. residua), but free of C. parvum infection, were used to investigate the recognition of C. parvum oocyst antigens by the Western blot procedure. Fractions in the 11.5-94 kDa range, as well as others with molecular masses over 94 kDa, were recognized by sera from rabbits. Sera collected during patency period showed low or moderate reaction with antigenic fractions in the 11.5-25 kDa range. However, 29, 58 and 71 to 75 kDa proteic fractions were moderately or strongly recognized even after rabbits finished oocyst excretion.

摘要

利用间接荧光抗体试验（IFAT）和蛋白质印迹法研究了微小隐孢子虫和艾美耳属球虫之间的免疫交叉反应性。采用艾美耳属球虫卵囊作为抗原，通过IFAT对37份来自无症状（无腹泻）牛的血清进行检测，这些牛具有已知的粪便学（球虫存在与否）和关于微小隐孢子虫的血清学数据。大多数血清（54%）在艾美耳属球虫卵囊表面周围显示出免疫荧光。同时，使用自然感染艾美耳属球虫（大型艾美耳球虫、肠艾美耳球虫和残余艾美耳球虫）但未感染微小隐孢子虫的兔血清样本，通过蛋白质印迹法研究对微小隐孢子虫卵囊抗原的识别。兔血清识别出分子量在11.5 - 94 kDa范围内的组分以及其他分子量超过94 kDa的组分。在排卵高峰期收集的血清与11.5 - 25 kDa范围内的抗原组分反应较弱或中等。然而，即使在兔停止排卵后，29、58以及71至75 kDa的蛋白质组分仍被适度或强烈识别。