无症状成年牛隐孢子虫卵囊及抗微小隐孢子虫IgG抗体的检测
Detection of oocysts and IgG antibodies to Cryptosporidium parvum in asymptomatic adult cattle.
作者信息
Lorenzo Lorenzo M J, Ares-Mazás E, Villacorta Martínez de Maturana I
机构信息
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, La Coruña, Spain.
出版信息
Vet Parasitol. 1993 Mar;47(1-2):9-15. doi: 10.1016/0304-4017(93)90171-i.
Infection by Cryptosporidium was detected in 94 (71.75%) asymptomatic adult cattle from 131 fecal samples examined microscopically. In two cases Cryptosporidium oocysts were observed which were distinctly larger (5.5-6.5 microns x 6.6-7.0 microns) than those we had seen in the majority of feces examined (4.0-4.5 microns x 4.0-4.5 microns) and these specimens were considered to be Cryptosporidium muris; it is possible that the other oocysts should be considered as Cryptosporidium parvum. The seroprevalence of IgG antibodies to Cryptosporidium was 63.35% as detected by indirect fluorescent antibody test (IFAT) and 51.41% by enzyme-linked immunosorbent assay (ELISA). In 27 cases, the presence of IgG antibodies to Cryptosporidium (as tested by IFAT and ELISA) in serum samples was correlated with oocyst excretion.
在通过显微镜检查的131份粪便样本中，检测到94头（71.75%）无症状成年牛感染了隐孢子虫。在两例中观察到隐孢子虫卵囊，其明显大于我们在大多数检查粪便中看到的卵囊（5.5 - 6.5微米×6.6 - 7.0微米，而大多数粪便中为4.0 - 4.5微米×4.0 - 4.5微米），这些样本被认为是鼠隐孢子虫；其他卵囊有可能应被视为微小隐孢子虫。通过间接荧光抗体试验（IFAT）检测，针对隐孢子虫的IgG抗体血清阳性率为63.35%，通过酶联免疫吸附测定（ELISA）为51.41%。在27例中，血清样本中针对隐孢子虫的IgG抗体（通过IFAT和ELISA检测）的存在与卵囊排泄相关。
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