氨基酸的气相-液相色谱法。以N-乙酰基、正丙基S-羧甲基半胱氨酸盐形式测定胱氨酸和半胱氨酸。

Gas-liquid chromatography of amino acids. Determination of cystine and cysteine as N-acetyl, n-propyl S-carboxymethylcysteinate.

作者信息

Moodie I M, George R D

出版信息

J Chromatogr. 1976 Sep 15;124(2):315-9. doi: 10.1016/s0021-9673(00)89746-0.

Abstract

A procedure is described for the derivatisation of S-carboxymethylcysteine yielding the corresponding N-acetyl, n-propyl S-carboxymethylcysteinate which is separated by gas chromatography form similar derivatives of other protein amino acids. Its quantitative determination provides a useful extension to a method for analysis of amino acids by gas chromatography and offers potential as a means of obviating errors in cystine (and/or cysteine) analysis arising from acid hydrolysis of proteins.

摘要

本文描述了一种S-羧甲基半胱氨酸的衍生化方法，该方法可生成相应的N-乙酰基、正丙基S-羧甲基半胱氨酸盐，它可通过气相色谱法与其他蛋白质氨基酸的类似衍生物分离。其定量测定为气相色谱法分析氨基酸的方法提供了有益的扩展，并有望作为一种避免因蛋白质酸水解而导致胱氨酸（和/或半胱氨酸）分析误差的手段。