头发硫氨基酸分析。

Hair sulfur amino acid analysis.

作者信息

Zahn H, Gattner H G

机构信息

Deutsches Wollforschungsinstitut an der RWTH Aachen e.V., Germany.

出版信息

EXS. 1997;78:239-58. doi: 10.1007/978-3-0348-9223-0_8.

DOI:10.1007/978-3-0348-9223-0_8

PMID:8962496

Abstract

This overview emphasizes present aspects of sulfur-containing amino acids in hair. A selection of analytical procedures to determine cystine, cysteine, S-sulfocysteine, cystine oxide, cysteic acid, lanthionine and lysinoalanine are presented. The methods relate to intact hair or partial and total hydrolysates and comprise chromatography, titration, colorimetry, polarography and spectroscopy. For the analysis of cysteine, cystine and cystine oxides, polarography and spectroscopy are the methods of choice. Cysteic acid, lanthionine and lysinoalanine are analysed by means of ion-exchange chromatography (Spackman et al., 1958) after total hydrolysis.

摘要

本综述着重介绍了头发中含硫氨基酸的当前研究情况。文中介绍了一系列用于测定胱氨酸、半胱氨酸、S-磺基半胱氨酸、胱氨酸氧化物、磺基丙氨酸、羊毛硫氨酸和赖氨酸丙氨酸的分析方法。这些方法适用于完整头发或部分及完全水解产物，包括色谱法、滴定法、比色法、极谱法和光谱法。对于半胱氨酸、胱氨酸和胱氨酸氧化物的分析，极谱法和光谱法是首选方法。完全水解后，通过离子交换色谱法（Spackman等人，1958年）分析磺基丙氨酸、羊毛硫氨酸和赖氨酸丙氨酸。