衣原体和淋病感染诊断方法的评估与评价。
An assessment and evaluation of methods for diagnosis of chlamydial and gonococcal infections.
作者信息
Chomvarin C, Chantarsuk Y, Thongkrajai P, Waropastrakul N, Tesana N
机构信息
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand.
出版信息
Southeast Asian J Trop Med Public Health. 1997 Dec;28(4):791-800.
Chlamydia trachomatis and Neisseria gonorrhoeae were studied in 350 females and 140 males attending the sexually transmitted disease clinic and AIDS Center, Khon Kaen zone 6 and the Division of Obstetrics and Gynecology, Khon Kaen Hospital. Chlamydia trachomatis infection was diagnosed by cell culture (CC), enzyme immunoassay (EIA) (Bioquest, NSW, Australia) and nucleic acid hybridization (PACE2 system: Gen-Probe, San Diego, Calif). It was found that the sensitivity, specificity and positive and negative predictive values in females were 95.7, 100.0, 100.0, 99.7% by the cell culture; 91.3, 99.1, 87.5, 99.4% by the EIA; and 78.3, 99.7, 94.7, 98.5% by the PACE2 respectively. Values of the same parameters in males were 83.3, 100.0, 100.0, 98.5% by the cell culture; 75.0, 99.2, 90.0, 97.7% by the EIA and 91.7, 100.0, 100.0, 99.2% by PACE2 respectively. The methods for detection of Neisseria gonorrhoeae infection were conventional culture, PACE2 test and the direct examination (Gram's stain). In females, the sensitivity, specificity and positive and negative predictive values of the conventional culture were 85.7, 100.0, 100.0, 99.7% and those of the PACE2 were 85.7, 99.1, 66.7, 99.7% respectively. In males, the values of the same parameters were 81.8, 100.0, 100.0, 100.0% by the conventional culture, 95.5, 100.0, 100.0 and 99.2% by the PACE2. The prevalence of chlamydial infection in females was 6.6% (23/350) and that in males was 8.6% (12/140). The prevalence of gonococcal infection in females was 2.0% (7/350) and in males was 15.7% (22/140). The co-infection of Chlamydia trachomatis and Neisseria gonorrhoeae in females was 0.9% (3/350) and no co-infection was found in males. It is concluded that cell culture is an appropriate method for detection of Chlamydia trachomatis in both genders, particularly in females. PACE 2 test is the best method for such detection in symptomatic males while EIA is a good method in females, particularly in symptomatic females. For gonococcal detection, PACE2 test is a sensitive, specific and alternative method to the conventional culture. It can be appropriately applied for the diagnosis of gonococcal infection, particularly in males.
对孔敬府第6区性病诊所及艾滋病中心、孔敬医院妇产科的350名女性和140名男性进行了沙眼衣原体和淋病奈瑟菌研究。沙眼衣原体感染通过细胞培养（CC）、酶免疫测定（EIA）（澳大利亚新南威尔士州Bioquest公司产品）和核酸杂交（PACE2系统：美国加利福尼亚州圣地亚哥Gen-Probe公司产品）进行诊断。结果发现，在女性中，细胞培养法的敏感性、特异性、阳性预测值和阴性预测值分别为95.7%、100.0%、100.0%、99.7%；EIA法分别为91.3%、99.1%、87.5%、99.4%；PACE2法分别为78.3%、99.7%、94.7%、98.5%。在男性中，相同参数的值细胞培养法分别为83.3%、100.0%、100.0%、98.5%；EIA法分别为75.0%、99.2%、90.0%、97.7%；PACE2法分别为91.7%、100.0%、100.0%、99.2%。淋病奈瑟菌感染的检测方法为传统培养法、PACE2试验和直接检查（革兰氏染色）。在女性中，传统培养法的敏感性、特异性、阳性预测值和阴性预测值分别为85.7%、100.0%、100.0%、99.7%，PACE2法分别为85.7%、99.
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