家蚕细胞质型多角体病毒8片段的核苷酸序列。

Nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 8.

作者信息

Hagiwara K, Tomita M, Kobayashi J, Miyajima S, Yoshimura T

机构信息

Department of Chemistry for Materials, Faculty of Engineering, Mie University, Japan.

出版信息

Biochem Biophys Res Commun. 1998 Jun 29;247(3):549-53. doi: 10.1016/s0006-291x(98)80004-1.

DOI:10.1016/s0006-291x(98)80004-1

PMID:9657006

Abstract

The segments 8 (S8) of the 10 double-stranded RNA genomes from Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) strains I and H were converted into cDNAs, amplified by PCR, and cloned. The nucleotide sequence analysis of the two full-length S8 cDNAs showed that the segments consist of 1328 nucleotides encoding putative proteins (p44) of 390 amino acids with molecular masses of about 44 kDa, which have glutamic acid-rich and proline-rich domains in their central regions. They had quite high identity with each other: about 98% in nucleotide and amino acid sequences. The recombinant p44 expressed in BmN4 cells using the baculovirus vector was detected by immunoblot analysis. p44 was also confirmed with the same antiserum to be present in BmCPV-infected midgut cells, but not in polyhedra, virus virions and uninfected midgut cells, indicating that p44 is expressed as a nonstructural protein of BmCPV.

摘要

将家蚕细胞质型多角体病毒（BmCPV）I株和H株的10个双链RNA基因组的第8节段（S8）逆转录成cDNA，通过聚合酶链反应（PCR）扩增并克隆。对两个全长S8 cDNA的核苷酸序列分析表明，这些节段由1328个核苷酸组成，编码推定的蛋白质（p44），该蛋白质含有390个氨基酸，分子量约为44 kDa，其中心区域富含谷氨酸和脯氨酸结构域。它们彼此之间具有相当高的同一性：核苷酸和氨基酸序列的同一性约为98%。使用杆状病毒载体在BmN4细胞中表达的重组p44通过免疫印迹分析进行检测。用相同的抗血清也证实p44存在于受BmCPV感染的中肠细胞中，但不存在于多角体、病毒粒子和未感染的中肠细胞中，这表明p44作为BmCPV的一种非结构蛋白表达。