Carsia R V, Tilly K I, Tilly J L
Department of Cell Biology, University of Medicine and Dentistry of New Jersey-School of Osteopathic Medicine, Stratford 08084-1489, USA.
Endocrine. 1997 Dec;7(3):377-81. doi: 10.1007/BF02801333.
The intact rat adrenal gland in short-term (3-h) organ culture may be amenable for the identification of factors involved in regulating adrenal cell apoptosis under defined conditions. In this model, culturing in the absence of trophic support (basal; control) triggered apoptosis in the intact rat adrenal gland; oligonucleosome formation, a measure of apoptosis, was 56.4-fold greater than that of glands snap-frozen at the start of incubation. Angiotensin II (Ang II) (100 nM) enhanced apoptosis by 67% over control. By contrast, adrenocorticotropin (ACTH) (100 nM) attenuated basal apoptosis by 59% and antagonized the enhanced apoptosis induced by Ang II back to the control level. Quartering of the glands enhanced basal oligonucleosome formation 182.2% greater than that of intact glands. Interestingly, quartering of the glands abolished the influences of Ang II and ACTH on apoptotic DNA fragmentation, but did not alter ACTH-induced corticosterone secretion. These data suggest that some level of gross adrenal structural information or compartmentalization, sufficiently disrupted by quartering, is required for the hormonal modulation of adrenal cell survival.
短期（3小时）器官培养中的完整大鼠肾上腺，可能适合用于确定在特定条件下参与调节肾上腺细胞凋亡的因素。在该模型中，在缺乏营养支持的情况下（基础培养；对照）培养会引发完整大鼠肾上腺的凋亡；凋亡的一个指标——寡核小体形成，比孵育开始时速冻的腺体高56.4倍。血管紧张素II（Ang II）（100 nM）使凋亡比对照增强67%。相比之下，促肾上腺皮质激素（ACTH）（100 nM）使基础凋亡减少59%，并将Ang II诱导的增强凋亡拮抗至对照水平。将腺体切成四分之一比完整腺体使基础寡核小体形成增加182.2%。有趣的是，将腺体切成四分之一消除了Ang II和ACTH对凋亡性DNA片段化的影响，但未改变ACTH诱导的皮质酮分泌。这些数据表明，肾上腺细胞存活的激素调节需要一定水平的大体肾上腺结构信息或分隔，而切成四分之一会充分破坏这种结构信息或分隔。
