在二甲基亚砜中用乙酸酐氧化的组织切片中多糖的选择性组织化学定位。

Selective histochemical localization of polysaccharides in tissue sections oxidized by acetic anhydride in dimethyl sulfoxide.

作者信息

Malinin G I

出版信息

J Histochem Cytochem. 1976 Sep;24(9):993-9. doi: 10.1177/24.9.965716.

DOI:10.1177/24.9.965716

Abstract

Oxidation of tissue sections by 25-30% (v/v) acetic anhydride (AA) in dimethyl sulfoxide (DMSO) resulted in facile induction of tissue carbonyls readily localized with Schiff's reagent and o-dianisidine but not with the 3-hydroxy-2-naphthoic acid hydrazide-tetraazotized diorthoanisidine method. Carbonyls generated by AA-DMSO oxidation were confined predomintly to substrates containing pyranosides. Oxidized furanosides, as represented by deoxyribonucleic acid and ribonucleic acid, gave only a residual color reaction. The AA-DMSO method possesses an advantage in that the oxidation of tissue polysaccharides does not proceed beyond the formation of carbonyly and is particularly suited for use after formol fixation.

摘要

在二甲基亚砜（DMSO）中用25 - 30%（v/v）的乙酸酐（AA）对组织切片进行氧化，可轻易诱导组织羰基的产生，这些羰基很容易用席夫试剂和邻联茴香胺定位，但不能用3 - 羟基 - 2 - 萘甲酰肼 - 四氮杂化二邻茴香胺法定位。由AA - DMSO氧化产生的羰基主要局限于含有吡喃糖苷的底物。以脱氧核糖核酸和核糖核酸为代表的氧化呋喃糖苷仅产生残留颜色反应。AA - DMSO方法的优点在于组织多糖的氧化不会超过羰基的形成阶段，特别适合在甲醛固定后使用。