Root Kustritz M V, Olson P N, Johnston S D, Root T K
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
J Am Anim Hosp Assoc. 1998 Jul-Aug;34(4):348-52. doi: 10.5326/15473317-34-4-348.
Percentage and types of morphological abnormalities found in canine spermatozoa were evaluated by three investigators using three stains (Giemsa-Wright stain [Diff-Quik], eosin Y/nigrosin [Hancock], and eosin B/nigrosin [Society for Theriogenology morphology stain] with conventional light microscopy, compared to phase contrast microscopy on unstained samples. The percentage of spermatozoa with abnormal heads, midpieces, and tails varied by technique and by investigator. Average percentages of morphologically normal spermatozoa were significantly higher in samples stained with Diff-Quik and samples examined by phase contrast microscopy than in samples stained with Hancock or Society for Theriogenology morphology stains. No effect of investigator on the percentage of morphologically normal spermatozoa was assessed. Results suggest that staining or preparation technique may alter the morphology of canine spermatozoa artifactually.
三位研究人员使用三种染色剂（吉姆萨 - 瑞特染色剂[Diff - Quik]、伊红Y/黑色素[汉考克染色法]和伊红B/黑色素[兽医学形态学协会染色法]），通过传统光学显微镜对犬精子中发现的形态异常的百分比和类型进行了评估，并与未染色样本的相差显微镜检查结果进行了比较。头部、中段和尾部异常的精子百分比因技术和研究人员而异。用Diff - Quik染色的样本以及通过相差显微镜检查的样本中形态正常精子的平均百分比显著高于用汉考克染色法或兽医学形态学协会染色法染色的样本。未评估研究人员对形态正常精子百分比的影响。结果表明，染色或制备技术可能会人为地改变犬精子的形态。
