Yamazaki Y, Oka S
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
J Chromatogr B Biomed Sci Appl. 1998 May 29;709(2):306-9. doi: 10.1016/s0378-4347(98)00073-5.
Staining with both enantiomers of an alpha-naphthyl ester plus a diazonium salt and comparing the color intensities given by the two enantiomers is a convenient method to evaluate the esterase stereoselectivity for that ester in two-dimensional electropherograms and tissue sections. Application of this method for rat liver has shown that (1) several esterases, e.g., one of pI 6.4 and Mr 118 kDa, are moderately stereoselective against alpha-naphthyl (R)-N-acetylalaninate and (R)-N-methoxycarbonylalaninate but strictly stereoselective against alpha-naphthyl (S)-N-methoxycarbonylvalinate, implying that esterase stereoselectivity may be inverted by changing the ester structure; and (2) these esterases are mainly contained in the hepatocytes around central veins.
用α-萘酯的两种对映体加上重氮盐进行染色，并比较两种对映体产生的颜色强度，是在二维电泳图和组织切片中评估该酯的酯酶立体选择性的便捷方法。将该方法应用于大鼠肝脏已表明：(1) 几种酯酶，例如一种pI为6.4、Mr为118 kDa的酯酶，对α-萘基 (R)-N-乙酰丙氨酸酯和 (R)-N-甲氧基羰基丙氨酸酯具有中等立体选择性，但对α-萘基 (S)-N-甲氧基羰基缬氨酸酯具有严格立体选择性，这意味着通过改变酯结构，酯酶立体选择性可能会反转；(2) 这些酯酶主要存在于中央静脉周围的肝细胞中。
