Carrère J, Guy-Crotte O, Figarella C
Groupe de Recherche sur les Glandes Exocrines, Faculté de Médecine, Marseille, France.
Clin Chim Acta. 1998 May 25;273(2):185-94. doi: 10.1016/s0009-8981(98)00043-6.
A direct sandwich immunoassay was developed to quantify the human reg protein, a non enzymatic pancreatic acinar protein the biological function of which remains elusive. Polystyrene balls were coated with specific IgG fraction as the first antibody and horseradish peroxidase labelled IgG was used as a second antibody. The linearity of the assay was good over a concentration range of 1.25-100 ng/ml and the good parallelism obtained between the standard and the assay dilution curves in serum and pancreatic juice indicates the absence of non-specific interfering reactions. Gel filtration of serum showed that the reg protein was eluted in the fractions corresponding to the proteins of around 25 kDa and that the chromatographic behaviour of the serum protein was identical to that of the purified pancreatic protein when added to serum. This assay was simple, specific, sensitive and reproducible and may permit the determination of low levels of reg protein in different biological fluids.
开发了一种直接夹心免疫测定法来定量检测人reg蛋白，这是一种非酶促胰腺腺泡蛋白，其生物学功能仍不清楚。将聚苯乙烯球用特异性IgG组分作为第一抗体进行包被，并使用辣根过氧化物酶标记的IgG作为第二抗体。该测定法在1.25-100 ng/ml的浓度范围内线性良好，并且在血清和胰液中标准曲线与测定稀释曲线之间获得的良好平行性表明不存在非特异性干扰反应。血清的凝胶过滤显示，reg蛋白在对应于约25 kDa蛋白质的级分中洗脱，并且当添加到血清中时，血清蛋白的色谱行为与纯化的胰腺蛋白相同。该测定法简单、特异、灵敏且可重复，可能允许测定不同生物体液中低水平的reg蛋白。
