与哺乳动物trp2转录本相关的牛trp同源物的结构和mRNA表达

Structure and mRNA expression of a bovine trp homologue related to mammalian trp2 transcripts.

作者信息

Wissenbach U, Schroth G, Philipp S, Flockerzi V

机构信息

Institut für Pharmakologie und Toxikologie der Universität des Saarlandes, Homburg/Saar, Germany.

出版信息

FEBS Lett. 1998 Jun 5;429(1):61-6. doi: 10.1016/s0014-5793(98)00561-4.

DOI:10.1016/s0014-5793(98)00561-4

PMID:9657384

Abstract

Mammalian homologues of the transient receptor potential (trp) gene product from Drosophila melanogaster function as Ca2+-selective or non-selective cation channels. Complementary DNA was isolated from a bovine testis cDNA library which encodes bovine trp2 (btrp2), a protein of 432 amino acid residues comprising four predicted transmembrane segments. Btrp2 mRNA is expressed in bovine testis, spleen and liver but not in brain, heart, adrenal gland or retina. In bovine testis expression of btrp2 mRNA is restricted to spermatocytes but is not present in spermatogonia, Leydig or Sertoli cells suggesting that btrp2 may contribute to the formation of ion channels in sperm cells.

摘要

来自黑腹果蝇的瞬时受体电位（trp）基因产物的哺乳动物同源物作为Ca2+选择性或非选择性阳离子通道发挥作用。从牛睾丸cDNA文库中分离出互补DNA，其编码牛trp2（btrp2），一种由432个氨基酸残基组成的蛋白质，包含四个预测的跨膜片段。Btrp2 mRNA在牛睾丸、脾脏和肝脏中表达，但在脑、心脏、肾上腺或视网膜中不表达。在牛睾丸中，btrp2 mRNA的表达仅限于精母细胞，而在精原细胞、睾丸间质细胞或支持细胞中不存在，这表明btrp2可能有助于精子细胞中离子通道的形成。