Oehlen L, Cross F R
The Rockefeller University, New York, NY 10021, USA.
FEBS Lett. 1998 Jun 5;429(1):83-8. doi: 10.1016/s0014-5793(98)00568-7.
The transcription factor Tec1 is involved in pseudohyphal differentiation and agar-invasive growth of Saccharomyces cerevisiae cells. The sole element in the TEC1 promoter that has thus far been shown to control Tec1 function is the filament response element. We find that the TEC1 promoter also contains several pheromone response element sequences which are likely to be functional: TEC1 transcription is induced by mating factor, cell cycle regulated and dependent on the Ste4, Ste18 and Ste5 components of the mating factor signal transduction pathway. Using alleles of the transcription factor Ste12 that are defective in DNA binding, transcriptional induction or cooperativity with other transcription factors, we find little correlation between TEC1 transcript levels and agar-invasive growth.
转录因子Tec1参与酿酒酵母细胞的假菌丝分化和琼脂侵袭性生长。迄今为止，TEC1启动子中唯一被证明可控制Tec1功能的元件是丝状反应元件。我们发现TEC1启动子还包含几个可能具有功能的信息素反应元件序列：TEC1转录由交配因子诱导，受细胞周期调控，并依赖于交配因子信号转导途径的Ste4、Ste18和Ste5组分。利用在DNA结合、转录诱导或与其他转录因子协同作用方面存在缺陷的转录因子Ste12的等位基因，我们发现TEC1转录水平与琼脂侵袭性生长之间几乎没有相关性。
