用对流免疫电泳法检测副结核分枝杆菌抗体。

Detection of antibodies to Mycobacterium johnei by counterimmunoelectrophoresis.

作者信息

Muhammed S I, Tadayon R A, Cheema A H

出版信息

Vet Rec. 1978 May 6;102(18):401-3. doi: 10.1136/vr.102.18.401.

DOI:10.1136/vr.102.18.401

Abstract

Counterimmunoelectrophoresis (CIE) was applied in the detection of antibodies to Mycobacterium johnei in 110 sheep, 11 goat and 31 cattle sera and compared to immunodiffusion (ID) test. One per cent Noble agar, 7 ml per slide of 5 cm x 10 cm; barbitone-tris buffer, mu = 0.03, pH 8.6; a constant current of 5 mA per slide and M johnei protoplasmic antigen at 4 mg per ml were found to impart high sensitivity to CIE and give rapid results. CIE detected 97 sheep, 11 goat and 31 cattle positive sera, a total of 139, as compared to 44, 11, 28 and 83 respectively, detected by ID. Strongly positive sera could be demonstrated within 30 minutes by CIE and the test was run for only 90 minutes while earliest reactions were not observed before 18 hours and some reactions took 144 hours to develop in ID test.

摘要

采用对流免疫电泳（CIE）检测了110份绵羊血清、11份山羊血清和31份牛血清中抗副结核分枝杆菌抗体，并与免疫扩散（ID）试验进行了比较。结果发现，每片5 cm×10 cm的载玻片加7 ml 1%的诺布尔琼脂、巴比妥- Tris缓冲液（离子强度μ = 0.03，pH 8.6）、每片载玻片5 mA的恒定电流以及每毫升含4 mg的副结核分枝杆菌原生质抗原有助于提高CIE的灵敏度并能快速得出结果。CIE检测出97份绵羊阳性血清、11份山羊阳性血清和31份牛阳性血清，共计139份阳性血清，而ID分别检测出44份、11份、28份和83份阳性血清。CIE可在30分钟内检测出强阳性血清，整个检测只需90分钟，而ID试验最早在18小时后才出现反应，有些反应则需144小时才显现出来。