Aguilar-Torres F G, Rytel M W, Kagan I G
Am J Trop Med Hyg. 1976 Sep;25(5):667-70. doi: 10.4269/ajtmh.1976.25.667.
Application of counterimmunoelectrophoresis (CIE) in detection of antibodies against Trypanosoma cruzi in sera of 180 subjects from an endemic area (Bolivia), and 50 subjects from a nonendemic area (U.S.A.) was carried out, and the results were compared to ones obtained with latex agglutination (LA) and indirect hemagglutination (IHA). The optimal conditions for CIE were determined to be as follows: barbitone buffer of pH 8.2 and ionic strength 0.05 M was used in the chambers and in preparing the 1% agarose plates. The wells in the plates were 5 mm in diameter and 3 mm apart. Electric current was applied at 500 volts and 25 mA for 60 minutes. T. cruzi antigen (Center for Disease Control) was used in a dilution of 1:2. The sera were studied undiluted. The study population comprised the following groups: LA+ IHA+ (group I); LA+ IHA- (group II); LA- IHA+ (group III); LA- IHA- (group IV); and the control group (group V). Each group was comprised of 50 sera, except for group II which was comprised of only 30 sera. Antibodies were detected by CIE in 94% of sera in group I, 40% in group II, 76% in group III and 6% in group IV. There were no positive reactions in group V. CIE was positive in 74% of sera from the combined LA+ serum groups (I and II), and in 85% of sera from the IHA+ groups (I and III). A good correlation between CIE and the other tests in the LA+ and IHA+ group (I), and the lack of false positive reactions in group V suggest that CIE is a good diagnostic method for detection of T. cruzi antibodies. However, this study showed that IHA is a more sensitive test in epidemiological surveys than CIE. The finding of 6% sera positive by CIE in group IV may indicate a lower specificity of the test.
应用对流免疫电泳（CIE）检测来自流行区（玻利维亚）的180名受试者和非流行区（美国）的50名受试者血清中抗克氏锥虫抗体，并将结果与乳胶凝集试验（LA）和间接血凝试验（IHA）的结果进行比较。确定CIE的最佳条件如下：在电泳槽中以及制备1%琼脂糖平板时使用pH 8.2、离子强度0.05M的巴比妥缓冲液。平板上的孔直径为5mm，间距为3mm。施加500伏电压和25毫安电流，持续60分钟。使用1:2稀释的克氏锥虫抗原（疾病控制中心）。血清不稀释进行检测。研究人群包括以下几组：LA+IHA+（第一组）；LA+IHA-（第二组）；LA-IHA+（第三组）；LA-IHA-（第四组）；以及对照组（第五组）。除第二组仅含30份血清外，每组均含50份血清。CIE检测到第一组94%的血清中有抗体，第二组40%，第三组76%，第四组6%。第五组无阳性反应。CIE在LA+血清组（第一组和第二组）74%的血清中呈阳性，在IHA+组（第一组和第三组）85%的血清中呈阳性。CIE与LA+和IHA+组（第一组）中的其他检测方法之间具有良好的相关性，且第五组无假阳性反应，这表明CIE是检测克氏锥虫抗体的良好诊断方法。然而，本研究表明，在流行病学调查中IHA比CIE更敏感。第四组中CIE检测到6%的血清呈阳性，这一结果可能表明该检测方法的特异性较低。
