Identification of linear DNA plasmids of the yeast Pichia pastoris.
作者信息
Banerjee H, Kopvak C, Curley D
机构信息
Department of Biology, Long Island University, Brooklyn Campus, New York, USA.
出版信息
Plasmid. 1998 Jul;40(1):58-60. doi: 10.1006/plas.1998.1341.
PMID:9657934
Abstract
Two DNA plasmids, approximately 11 and 8 kb in size, have been identified in a strain of the yeast Pichia pastoris (Northern Regional Research Laboratories No. Y4290). The plasmids are resistant to RNase A and lambda exonuclease, but are sensitive to digestion by DNase I, suggesting that they are linear and double-stranded DNA with 5'-protected ends. A restriction map has been constructed for the 11-kb plasmid, confirming that it is linear.
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