Colberg-Poley A M, Huang L, Soltero V E, Iskenderian A C, Schumacher R F, Anders D G
Children's Research Institute, Children's National Medical Center, Washington, DC 20010,
Virology. 1998 Jul 5;246(2):400-8. doi: 10.1006/viro.1998.9212.
Transient complementation of human cytomegalovirus (HCMV) oriLyt DNA replication in permissive human diploid cells expressing replication genes under native promoters requires its UL36-38 gene products. Two of the immediate early (IE) proteins encoded by this locus, pUL37x1 and, to a lesser extent, gpUL37, activated expression of HCMV early gene promoter constructions. The other IE protein encoded by the UL36-38 locus, pUL36, and the early product, pUL38, did not transactivate the HCMV early promoter constructions under similar conditions. The acidic domain, common to both pUL37x1 and gpUL37, is required for activation of HCMV early promoter constructions. Conversely, gpUL37 sequences downstream of amino acid 199 are not required for transactivation of viral early promoters. Taken together, these results suggest that the requirement for UL36-38 products for HCMV DNA replication results, at least in part, from the requirement of the transactivation of HCMV early DNA replication promoters by pUL37x1 and, to a lesser extent, by gpUL37 and that the acidic domain is critical for this activity.
在天然启动子控制下表达复制基因的允许性人二倍体细胞中，人巨细胞病毒（HCMV）oriLyt DNA复制的瞬时互补需要其UL36 - 38基因产物。该基因座编码的两种立即早期（IE）蛋白，pUL37x1以及程度稍弱的gpUL37，激活了HCMV早期基因启动子构建体的表达。UL36 - 38基因座编码的另一种IE蛋白pUL36和早期产物pUL38，在类似条件下并未反式激活HCMV早期启动子构建体。pUL37x1和gpUL37共有的酸性结构域是激活HCMV早期启动子构建体所必需的。相反，病毒早期启动子反式激活不需要氨基酸199下游的gpUL37序列。综上所述，这些结果表明，HCMV DNA复制对UL36 - 38产物的需求至少部分源于pUL37x1以及程度稍弱的gpUL37对HCMV早期DNA复制启动子反式激活的需求，并且酸性结构域对此活性至关重要。
