空肠弯曲菌鞭毛钩蛋白FlgE的中央表面暴露区域在菌株间表现出高度变异性。
The central, surface-exposed region of the flagellar hook protein FlgE of Campylobacter jejuni shows hypervariability among strains.
作者信息
Lüneberg E, Glenn-Calvo E, Hartmann M, Bär W, Frosch M
机构信息
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Würzburg, Germany.
出版信息
J Bacteriol. 1998 Jul;180(14):3711-4. doi: 10.1128/JB.180.14.3711-3714.1998.
Abstract
In a previous study, we observed that monoclonal antibodies raised against the hook protein FlgE of Campylobacter jejuni LIO 36, isolate 5226, bound exclusively to this strain. The aim of this study was to elucidate the molecular basis for these binding specificities. The hook protein-encoding gene flgE of C. jejuni was cloned in Escherichia coli and sequenced. The flgE genes of four additional C. jejuni strains were amplified by PCR and also sequenced. Comparison of the deduced amino acid sequences revealed a high degree of variability in the central parts of the FlgE proteins among the strains, including variable and hypervariable domains. These findings may indicate a selective pressure of C. jejuni hosts, forcing the bacteria to generate variations in surface-exposed antigenic determinants.
摘要
在之前的一项研究中，我们观察到针对空肠弯曲菌LIO 36（菌株5226）的钩蛋白FlgE产生的单克隆抗体仅与该菌株结合。本研究的目的是阐明这些结合特异性的分子基础。空肠弯曲菌的钩蛋白编码基因flgE在大肠杆菌中克隆并测序。另外四个空肠弯曲菌菌株的flgE基因通过PCR扩增并测序。推导的氨基酸序列比较显示，菌株间FlgE蛋白中部存在高度变异性，包括可变区和高变区。这些发现可能表明空肠弯曲菌宿主存在选择性压力，迫使细菌在表面暴露的抗原决定簇中产生变异。
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