人类呼吸道合胞病毒融合糖蛋白的抗原结构
Antigenic structure of human respiratory syncytial virus fusion glycoprotein.
作者信息
López J A, Bustos R, Orvell C, Berois M, Arbiza J, García-Barreno B, Melero J A
机构信息
Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain.
出版信息
J Virol. 1998 Aug;72(8):6922-8. doi: 10.1128/JVI.72.8.6922-6928.1998.
Abstract
New series of escape mutants of human respiratory syncytial virus were prepared with monoclonal antibodies specific for the fusion (F) protein. Sequence changes selected in the escape mutants identified two new antigenic sites (V and VI) recognized by neutralizing antibodies and a group-specific site (I) in the F1 chain of the F molecule. The new epitopes, and previously identified antigenic sites, were incorporated into a refined prediction of secondary-structure motifs to generate a detailed antigenic map of the F glycoprotein.
摘要
利用针对融合（F）蛋白的单克隆抗体制备了一系列新的人呼吸道合胞病毒逃逸突变体。在逃逸突变体中选择的序列变化确定了两个新的被中和抗体识别的抗原位点（V和VI）以及F分子F1链中的一个组特异性位点（I）。这些新表位和先前确定的抗原位点被纳入对二级结构基序的精细预测中，以生成F糖蛋白的详细抗原图谱。
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