结合型和游离型胆汁酸的硅酸薄层色谱法。
Silicic acid thin-layer chromatography of conjugated and free bile acids.
作者信息
Chavez M N, Krone C L
出版信息
J Lipid Res. 1976 Sep;17(5):545-7.
PMID:965845
Abstract
The separation of free bile acids, taurine conjugates, and glycine conjugates from one another was achieved by thin-layer chromatography, using a solvent system of isopropanol glacial acetic acid 93:7 in a solvent system of hexane-methylethylketone-glacial acetic acid 56:36:8 (v/v), it was possible to separate cholic, chenodeoxycholic, deoxycholic, and lithocholic acids. Application to a biological bile sample was demonstrated.
摘要
通过薄层色谱法实现了游离胆汁酸、牛磺酸共轭物和甘氨酸共轭物之间的分离，使用异丙醇-冰醋酸93:7的溶剂系统，在己烷-甲基乙基酮-冰醋酸56:36:8（v/v）的溶剂系统中，可以分离胆酸、鹅去氧胆酸、脱氧胆酸和石胆酸。还展示了该方法在生物胆汁样本中的应用。
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