Hillsley K, Mawe G M
Department of Anatomy and Neurobiology, University of Vermont, Burlington 05405, USA.
Neurogastroenterol Motil. 1998 Jun;10(3):235-44. doi: 10.1046/j.1365-2982.1998.00101.x.
Sphincter of Oddi (SO) ganglia are comprised of two main types of neurones based either on their electrical or neurochemical properties. This study investigated whether any correlation exists between the electrical and neurochemical properties of these cells. SO neurones were characterized electrically as either Tonic or Phasic cells, labelled with neurobiotin, fixed, and processed for beta-nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-DA) staining and choline acetyltransferase immuno-reactivity to identify whether electrically characterized neurones were nitrergic or cholinergic. A total of 119 cells were analysed in this manner; 45% of cells were Tonic and 37% were Phasic. An equivalent number of Tonic (58.1%, 18/31) and Phasic cells (60%, 21/35) were choline acetyltransferase (ChAT) positive. Three of 34 Phasic cells were NADPH-DA positive, whereas 11/33 Tonic cells were NADPH-DA positive. In none of the preparations was ChAT immunoreactivity and NADPH-DA reactivity ever observed in the same neurone. Calretinin immunoreactivity was present in a subpopulation of both Tonic and Phasic neurones. No correlation was observed between the direction of axon projections and the electrophysiological or neurochemical properties of the cell. These results suggest that there is a lack of correlation between the electrical properties and the neurochemical content of SO neurones. Various explanations for these findings are discussed.
奥迪括约肌（SO）神经节由基于电特性或神经化学特性的两种主要类型的神经元组成。本研究调查了这些细胞的电特性与神经化学特性之间是否存在任何相关性。SO神经元在电生理上被表征为紧张性或相位性细胞，用神经生物素标记，固定后进行β-烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-DA）染色和胆碱乙酰转移酶免疫反应性检测，以确定电生理表征的神经元是一氧化氮能还是胆碱能。总共以这种方式分析了119个细胞；45%的细胞为紧张性，37%为相位性。同等数量的紧张性细胞（58.1%，18/31）和相位性细胞（60%，21/35）胆碱乙酰转移酶（ChAT）呈阳性。34个相位性细胞中有3个NADPH-DA呈阳性，而33个紧张性细胞中有11个NADPH-DA呈阳性。在所有制剂中，从未在同一神经元中观察到ChAT免疫反应性和NADPH-DA反应性。钙视网膜蛋白免疫反应性存在于紧张性和相位性神经元的一个亚群中。未观察到轴突投射方向与细胞的电生理或神经化学特性之间存在相关性。这些结果表明，SO神经元的电特性与神经化学含量之间缺乏相关性。讨论了对这些发现的各种解释。
