Li Z S, Furness J B
Department of Anatomy & Cell Biology, University of Melbourne, Parkville, VIC 3052, Australia.
Cell Tissue Res. 1998 Oct;294(1):35-43. doi: 10.1007/s004410051154.
Antibodies against choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) were used to determine whether neurons that have previously been identified as intrinsic primary afferent neurons in the guinea-pig small intestine have a cholinergic phenotype. Cell bodies of primary afferent neurons in the myenteric plexus were identified by their calbindin immunoreactivity and those in the submucous plexus by immunoreactivity for substance P. High proportions of both were immunoreactive for ChAT, viz. 98% of myenteric calbindin neurons and 99% of submucosal substance P neurons. ChAT immunoreactivity also occurred in all nerve cell bodies immunoreactive for calretinin and substance P in the myenteric plexus, but in only 16% of nerve cells immunoreactive for nitric oxide synthase. VAChT immunoreactivity was in the majority of calbindin-immunoreactive varicosities in the myenteric ganglia, submucous ganglia and mucosa and also in the majority of the varicosities of neurons that were immunoreactive for calretinin and somatostatin and that had been previously established as being cholinergic. We conclude that the intrinsic primary afferent neurons are cholinergic and that they may release transmitter from their sensory endings in the mucosa.
利用抗胆碱乙酰转移酶（ChAT）和囊泡乙酰胆碱转运体（VAChT）的抗体，来确定在豚鼠小肠中先前被鉴定为内在初级传入神经元的那些神经元是否具有胆碱能表型。肌间神经丛中初级传入神经元的胞体通过其钙结合蛋白免疫反应性来识别，而黏膜下神经丛中的则通过对P物质的免疫反应性来识别。两者中很大比例的神经元对ChAT呈免疫反应性，即98%的肌间钙结合蛋白神经元和99%的黏膜下P物质神经元。ChAT免疫反应性也出现在肌间神经丛中所有对钙视网膜蛋白和P物质呈免疫反应性的神经细胞体中，但仅出现在16%对一氧化氮合酶呈免疫反应性的神经细胞中。VAChT免疫反应性存在于肌间神经节、黏膜下神经节和黏膜中大多数对钙结合蛋白呈免疫反应性的曲张体中，也存在于大多数对钙视网膜蛋白和生长抑素呈免疫反应性且先前已确定为胆碱能的神经元的曲张体中。我们得出结论，内在初级传入神经元是胆碱能的，并且它们可能从其在黏膜中的感觉末梢释放递质。
