Huh Y O, Huck L
Division of Laboratory Medicine, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Clin Lab Sci. 1992 Jan-Feb;5(1):25-7.
The application of flow cytometry in evaluation of neoplasms is reviewed, with emphasis on DNA content analysis and surface-membrane antigen analysis. By measuring total nuclear DNA content one can determine the ploidy status and S-phase fraction of tumor cells. Aneuploidy, an abnormal DNA content, is a marker of malignancy and often a predictor of poor prognosis in a number of tumor categories. S-phase fraction reflects the proliferative activity of the tumor and is therefore related to the aggressiveness of the tumor. Surface-membrane antigen analysis of hematologic malignancies using monoclonal antibodies is useful in determining lineage and differentiation of tumor cells. This information is helpful in diagnosis and monitoring of patients and also has prognostic values. The flow cytometry is a new tool for the management of cancer patients because of its role in diagnosis, monitoring, and prognosis of tumors.
本文综述了流式细胞术在肿瘤评估中的应用，重点介绍了DNA含量分析和表面膜抗原分析。通过测量总核DNA含量，可以确定肿瘤细胞的倍体状态和S期分数。非整倍体是一种异常的DNA含量，是恶性肿瘤的标志物，并且在许多肿瘤类型中通常是预后不良的预测指标。S期分数反映了肿瘤的增殖活性，因此与肿瘤的侵袭性相关。使用单克隆抗体对血液系统恶性肿瘤进行表面膜抗原分析，有助于确定肿瘤细胞的谱系和分化。这些信息有助于患者的诊断和监测，也具有预后价值。由于流式细胞术在肿瘤诊断、监测和预后方面的作用，它是癌症患者管理的一种新工具。
