Guarnieri D J, Dodson G S, Simon M A
Department of Biological Sciences, Stanford University, California 94305, USA.
Mol Cell. 1998 May;1(6):831-40. doi: 10.1016/s1097-2765(00)80082-9.
Mutation of the Src64 gene of Drosophila results in ovarian ring canal defects and reduced female fertility. We used a dosage-sensitive modifier screen to search for downstream components of the SRC64 signaling pathway. We show that mutations affecting Tec29, an essential gene encoding a member of the Tec family of protein tyrosine kinases, dominantly enhance the Src64 ring canal phenotype. Loss of Tec29 function in the female germline results in a phenotype strikingly similar to that caused by the loss of Src64 function. In each case, the ring canals are reduced in size and phosphotyrosine content. We further demonstrate that TEC29 localizes to the ring canal, and this subcellular localization requires Src64 function. These data suggest that TEC29 is a downstream target of SRC64, and that regulating TEC29 localization during ring canal growth may be a crucial SRC64 function.
果蝇Src64基因的突变会导致卵巢环管缺陷并降低雌性生育力。我们使用了剂量敏感型修饰基因筛选来寻找SRC64信号通路的下游组分。我们发现，影响Tec29（一个编码蛋白酪氨酸激酶Tec家族成员的必需基因）的突变会显性增强Src64环管表型。雌性生殖系中Tec29功能的丧失导致的表型与Src64功能丧失所引起的表型惊人地相似。在每种情况下，环管的大小和磷酸酪氨酸含量都会降低。我们进一步证明，TEC29定位于环管，并且这种亚细胞定位需要Src64的功能。这些数据表明，TEC29是SRC64的下游靶点，并且在环管生长过程中调节TEC29的定位可能是SRC64的一项关键功能。
