通过溴脱氧尿苷免疫组织化学评估光动力疗法的抗肿瘤效果。

The anti-tumor effect of photodynamic therapy evaluated by bromodeoxyuridine immunohistochemistry.

作者信息

Uehara M, Inokuchi T, Sano K, Pe M B

机构信息

Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Japan.

出版信息

Int J Oral Maxillofac Surg. 1998 Jun;27(3):204-8. doi: 10.1016/s0901-5027(98)80011-8.

DOI:10.1016/s0901-5027(98)80011-8

PMID:9662014

Abstract

The anti-tumor effect of photodynamic therapy (PDT) on mouse tumors was evaluated with bromodeoxyuridine (BrdU) immunohistochemistry. BrdU was injected into the mice intraperitoneally (40 mg/kg body weight). Immediately after injection of BrdU, PDT using a photosensitizing drug (hematoporphyrin oligomers: 20 mg/kg body weight) was carried out on the experimental group but not on the control group. BrdU labeling indices (LIs) of the tumor cells close to blood vessels and adjacent to the surrounding normal tissue were investigated. In the tumor cells close to blood vessels, the LIs of the experimental group were significantly lower than those of the control group. As for the tumor cells adjacent to the surrounding normal tissue, the LIs of the experimental group were similar to those of the control group. Thus, the effect of PDT was significant in the tumor cells close to the blood vessels, while the tumor cells adjacent to the surrounding normal tissue resisted PDT.

摘要

采用溴脱氧尿苷（BrdU）免疫组织化学方法评估光动力疗法（PDT）对小鼠肿瘤的抗肿瘤作用。将BrdU腹腔注射到小鼠体内（40mg/kg体重）。注射BrdU后立即对实验组进行使用光敏药物（血卟啉寡聚物：20mg/kg体重）的PDT治疗，而对照组不进行治疗。研究靠近血管以及与周围正常组织相邻的肿瘤细胞的BrdU标记指数（LIs）。在靠近血管的肿瘤细胞中，实验组的LIs显著低于对照组。至于与周围正常组织相邻的肿瘤细胞，实验组的LIs与对照组相似。因此，PDT对靠近血管的肿瘤细胞效果显著，而与周围正常组织相邻的肿瘤细胞对PDT具有抗性。