Müller R, Van Campenhout H, Van Damme B, Van Der Perre G, Dequeker J, Hildebrand T, Rüegsegger P
Orthopedic Biomechanics Laboratory, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA.
Bone. 1998 Jul;23(1):59-66. doi: 10.1016/s8756-3282(98)00068-4.
As a part of the European Union BIOMED I study "Assessment of Bone Quality in Osteoporosis," the trabecular structure of transiliac bone biopsies was assessed by conventional histomorphometry and by three-dimensional microcomputed tomography (microCT). Sixty-three cylindrical human transiliac bone specimens were obtained post mortem from 27 women and 36 men (age 23-92 years) and evaluated morphometrically in this study. For the conventional histomorphometric analysis, a pair of four undecalcified sections were prepared from two regions in the mediolateral plane of the bone cores. The sections were stained with the von Kossa technique to obtain high-contrast two-dimensional images. Prior to the histologic sectioning the samples were measured with a desktop microCT, providing a 14 microm nominal resolution. The morphometric parameters computed by both methods in two or three dimensions, respectively, were bone volume density (BV/TV), bone surface density (BS/TV), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp). Results showed highly significant (p < 0.0001) correlations between conventional histology and microCT for BV/TV (r = 0.93), BS/TV (r = 0.91), Tb.Th (r = 0.84), and Tb.Sp (r = 0.91). The mean percentage differences between histology and microCT ranged from 2.5% (BV/TV) to 6.1% (Tb.Th). The high correlations and the low differences in terms of absolute numbers between conventional histomorphometric and microtomographic analysis are very promising for the use of micro-tomographic imaging. MicroCT is a nondestructive, fast, and very precise procedure that allows the measurement of cancellous and compact bone in unprocessed biopsies or small bones, as well as a fully automatic determination of three-dimensional morphometric indices.
作为欧盟BIOMED I研究“骨质疏松症骨质量评估”的一部分，通过传统组织形态计量学和三维显微计算机断层扫描（显微CT）对经髂骨活检的小梁结构进行了评估。从27名女性和36名男性（年龄23 - 92岁）尸检后获取了63个圆柱形人体经髂骨标本，并在本研究中进行了形态计量学评估。对于传统组织形态计量学分析，从骨芯中外侧平面的两个区域制备了一对四张未脱钙切片。切片用冯·科萨技术染色以获得高对比度二维图像。在组织学切片之前，用台式显微CT对样本进行测量，提供14微米的标称分辨率。分别通过两种方法在二维或三维中计算的形态计量学参数为骨体积密度（BV/TV）、骨表面密度（BS/TV）、小梁厚度（Tb.Th）和小梁间距（Tb.Sp）。结果显示，传统组织学与显微CT在BV/TV（r = 0.93）、BS/TV（r = 0.91）、Tb.Th（r = 0.84）和Tb.Sp（r = 0.91）方面具有高度显著（p < 0.0001）的相关性。组织学与显微CT之间的平均百分比差异范围为2.5%（BV/TV）至6.1%（Tb.Th）。传统组织形态计量学和显微断层扫描分析之间在绝对数值方面的高相关性和低差异对于显微断层成像的应用非常有前景。显微CT是一种无损、快速且非常精确的程序，可用于测量未处理活检组织或小骨中的松质骨和密质骨，以及全自动确定三维形态计量学指标。
