Kovaleva T S, Pantiukhina A N, Petrov V F
Zh Mikrobiol Epidemiol Immunobiol. 1998 Mar-Apr(2):65-8.
Species-specific antigenic erythrocyte diagnosticum (SAED) for the detection of antibodies to R.prowazekii was developed. The cells used as the basis for the preparation of the antigenic erythrocyte diagnosticum were formulated and tanned sheep red blood cells (FT-SRBC). Original indication antigens, solubilized with Triton X-100 from common R.prowazekii antigen for the complement fixation test, the antigen neutralization test and the fluorescent analysis, were used as sensitin. The optimum parameters for sensitizing FT-SRBC were established. The SAED thus obtained was stabilized by the method of lyophilization. In a separate series of experiments the specificity and activity of SAED were studied in comparison with those of preparations obtained with the use of different sensitins. The newly developed SAED was shown to be highly specific.
开发了用于检测抗普氏立克次体抗体的种特异性抗原红细胞诊断制剂（SAED）。用作制备抗原红细胞诊断制剂基础的细胞是经鞣酸处理的绵羊红细胞（FT-SRBC）。用Triton X-100从用于补体结合试验、抗原中和试验和荧光分析的普通普氏立克次体抗原中溶解得到的原始指示抗原用作致敏素。确定了FT-SRBC致敏的最佳参数。通过冻干法使由此获得的SAED稳定。在另一系列实验中，与使用不同致敏素获得的制剂相比，研究了SAED的特异性和活性。新开发的SAED显示出高度特异性。
