Vaisman I Sh, Barban P S, Sazonova L A, Misenzhnikov A V, Mirskiĭ V Ia
Zh Mikrobiol Epidemiol Immunobiol. 1978 Jul(7):136-40.
A study on ultra-thin sections was made of the preparations of agglutinate produced during the reaction of the immunoglobulin erythrocytic diagnostic agent with dry corpuscular Rickettsia prowazeki antigen, fluoresceine isothiocyanate labeled, and also SRBC used for the preparation of the diagnostic agent after formalinization, tannin treatment, sensitization with hyperimmune horse serum immunoglobulins and lyophilization, respectively. Formalin and tannin treatment of erythrocytes failed to be reflected on the ultrastructure of their cellular membranes; the treatment with hemosensitin was accompanied by the appearance of spheroid protrusions of the erythrocyte cytoplasmic membrane with the preservation of its three-layer structure. Specific interaction of sensitized erythrocytes with the antigen corpuscles was expressed morphologically in their apposition or connection through a gap of 20--30 nm.
对免疫球蛋白红细胞诊断剂与荧光素异硫氰酸酯标记的干燥普氏立克次体抗原反应过程中产生的凝集物制剂，以及分别经福尔马林固定、单宁处理、用超免疫马血清免疫球蛋白致敏和冻干后用于制备诊断剂的绵羊红细胞制剂制作了超薄切片研究。红细胞经福尔马林和单宁处理后，其细胞膜的超微结构未受影响；用血细胞致敏素处理后，红细胞细胞质膜出现球状突起，但其三层结构得以保留。致敏红细胞与抗原颗粒的特异性相互作用在形态上表现为它们并列或通过20 - 30纳米的间隙相连。
