Chen Y J, Shih L S, Chen Y M
Division of Preventive Medicine, Institute of Public Health, School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
Int J Oncol. 1998 Aug;13(2):249-54.
The mRNA level of CDKN2 (p16), retinoblastoma (Rb) and p53 in cancerous and non-cancerous tissue samples were compared using a semi-quantitative reverse-transcription-polymerase chain reaction method. The mean CDKN2 mRNA level of 10 gastric cancer specimens was significantly higher than that of their non-cancerous tissue samples (p=0.039). In contrast, the mRNA levels of Rb and p53 in gastric cancer tissues were both significantly lower than those in their non-cancerous tissue samples. Furthermore, both mRNA ratios of Rb/CDKN2 and p53/CDKN2 were significantly decreased in gastric cancer tissues. Therefore, suppression of both Rb and p53 and overexpression of CDKN2 may be associated with the tumorigenesis of gastric cancer.
采用半定量逆转录聚合酶链反应方法比较癌组织和非癌组织样本中CDKN2（p16）、视网膜母细胞瘤（Rb）和p53的mRNA水平。10例胃癌标本的CDKN2 mRNA平均水平显著高于其非癌组织样本（p = 0.039）。相反，胃癌组织中Rb和p53的mRNA水平均显著低于其非癌组织样本。此外，胃癌组织中Rb/CDKN2和p53/CDKN2的mRNA比值均显著降低。因此，Rb和p53的抑制以及CDKN2的过表达可能与胃癌的发生有关。
