Li You-ming, Yu Zhao-hui, Li Lan, Xu Ping, Li Lin, Zhang Bao-feng, Fang Jing, Zhou Qiong, Hu Ying, Gao Heng-jun
First Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310003, China.
Zhonghua Yi Xue Za Zhi. 2006 Apr 11;86(14):939-42.
To detect the expression of tumor suppressor protein p53, cyclin-dependent kinase inhibitor p16, and cyclooxygenase 2 (COX-2) in pancreatic cancer by tissue microarray and investigate the correlation among these three genes.
104 specimens of tissues, including pancreatic cancer tissue, non-cancer tissues not more than 1.5 cm from the cancer, and normal tissues, underwent microarray examination and immunohistochemistry to detect the expression of p53, p16, and COX-2. The correlation among these 3 genes was analyzed.
p53, p16, and COX-2 were all significantly highly expressed in the cancerous tissues in comparison with other tissues. P53 and p16 were both significantly correlated with COX-2 (both P < 0.05), however, there was not a correlation between p53 and p16 (P > 0.05). There was a reciprocal relationship between p53 and COX-2 (P < 0.05, OR = 19.686) influencing the pathogenesis of pancreatic cancer.
Tissue microarray technique is effective method to detect multiple gene protein expression. Pathogenesis of pancreatic cancer is associated with p53, p16, and COX-2.
采用组织芯片检测胰腺癌中抑癌蛋白p53、细胞周期蛋白依赖性激酶抑制剂p16和环氧合酶2（COX-2）的表达，并探讨这三个基因之间的相关性。
对104份组织标本进行芯片检测和免疫组化，这些标本包括胰腺癌组织、距癌组织不超过1.5 cm的癌旁组织和正常组织，以检测p53、p16和COX-2的表达。分析这3个基因之间的相关性。
与其他组织相比，p53、p16和COX-2在癌组织中均显著高表达。P53和p16均与COX-2显著相关（均P<0.05），然而，p53与p16之间无相关性（P>0.05）。p53与COX-2之间存在相互关系（P<0.05，OR=19.686），影响胰腺癌的发病机制。
组织芯片技术是检测多种基因蛋白表达的有效方法。胰腺癌的发病机制与p53、p16和COX-2有关。
