Brauer M M, Burnstocks G
División Biologia Celular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Int J Dev Neurosci. 1998 Feb;16(1):29-39. doi: 10.1016/s0736-5748(97)00047-6.
The expression of beta-adrenoceptors in the rat uterus has been analysed during the peripubertal transition and following acute and chronic oestradiol treatment during prepubertal development. The distribution and density of beta-adrenoceptors was assessed autoradiographically on cryostat tissue sections using [3H]-dihydroalprenolol ([3H]-DHA). Binding sites were localised in all ages and experimental situations examined and showed the following intensity of labelling: endometrial epithelium > longitudinal muscle layer > circular myometrial layer > endometrial stroma. Competition experiments with the selective antagonists ICI 118,551 and atenolol, showed that most of the beta-adrenoceptors in the uterus belong to the beta(2) receptor subclass. In prepubertal animals, the density of [3H]-DHA binding sites was extremely low. Following puberty the density of binding sites showed a generalised increase. Acute administration of oestradiol at the end of the prepubertal period provoked an increase in the density of [3H]-DHA binding sites in all uterine regions, but the levels of labelling were lower than in peripubertal animals at proestrus and oestrus. Following chronic oestrogen treatment during postnatal development, oestradiol increased further the density of [3H]-DHA binding sites. Results are discussed considering both the endocrine and neural changes accompanying puberty and oestradiol treatment.
在青春期前后的过渡期以及青春期前发育期间急性和慢性雌二醇治疗后，对大鼠子宫中β-肾上腺素能受体的表达进行了分析。使用[3H]-二氢阿普洛尔（[3H]-DHA），通过放射自显影法在低温恒温器组织切片上评估β-肾上腺素能受体的分布和密度。在所检查的所有年龄和实验情况下，结合位点均有定位，并显示出以下标记强度：子宫内膜上皮>纵肌层>环形肌层>子宫内膜基质。用选择性拮抗剂ICI 118,551和阿替洛尔进行的竞争实验表明，子宫中的大多数β-肾上腺素能受体属于β(2)受体亚类。在青春期前动物中，[3H]-DHA结合位点的密度极低。青春期后，结合位点的密度普遍增加。在青春期前阶段结束时急性给予雌二醇会导致所有子宫区域中[3H]-DHA结合位点的密度增加，但标记水平低于发情前期和发情期的青春期前后动物。在出生后发育期间进行慢性雌激素治疗后，雌二醇进一步增加了[3H]-DHA结合位点的密度。结合青春期和雌二醇治疗伴随的内分泌和神经变化对结果进行了讨论。
