Fujioka S, Noguchi T, Yokota T, Takatsuto S, Yoshida S
Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Phytochemistry. 1998 Jun;48(4):595-9. doi: 10.1016/s0031-9422(98)00065-x.
From the seeds and siliques of Arabidopsis thaliana, six brassinosteroids, brassinolide, castasterone, typhasterol, 6-deoxocastasterone, 6-deoxotyphasterol and 6-deoxoteasterone, were identified by GC-mass spectrometry or GC-selected ion monitoring. As the occurrence of castasterone, typhasterol, 6-deoxocastasterone and 6-deoxotyphasterol in the shoots of A. thaliana has already been reported, this study provides evidence for the occurrence of the above four brassinosteroids in different organs, seeds and siliques, and the first evidence for the occurrence of brassinolide and 6-deoxoteasterone in A. thaliana. All brassinosteroids identified in this study belong to important components of both the early and late C-6 oxidation pathways, which were established in the cultured cells of Catharanthus roseus. This suggests that both pathways are operating in A. thaliana to produce the most biologically active brassinosteroid, brassinolide, which is responsible for growth and development of the plant.
通过气相色谱-质谱联用仪或气相色谱-选择离子监测法，从拟南芥的种子和角果中鉴定出了六种油菜素甾醇，即油菜素内酯、菜甾酮、typhasterol、6-脱氧菜甾酮、6-脱氧typhasterol和6-脱氧茶甾酮。由于菜甾酮、typhasterol、6-脱氧菜甾酮和6-脱氧typhasterol在拟南芥地上部分的存在情况已有报道，本研究为上述四种油菜素甾醇在不同器官、种子和角果中的存在提供了证据，同时也首次证明了油菜素内酯和6-脱氧茶甾酮在拟南芥中的存在。本研究鉴定出的所有油菜素甾醇均属于长春花培养细胞中建立的早期和晚期C-6氧化途径的重要组成部分。这表明这两条途径在拟南芥中均发挥作用，以产生对植物生长发育起关键作用的生物活性最强的油菜素甾醇——油菜素内酯。
