采用酶联免疫吸附测定法和蛋白质印迹分析法测定内分泌紊乱患者的抗垂体抗体。

Determination of antipituitary antibody in patients with endocrine disorders by enzyme-linked immunosorbent assay and Western blot analysis.

作者信息

Yabe S, Kanda T, Hirokawa M, Hasumi S, Osada M, Fukumura Y, Kobayashi I

机构信息

Department of Laboratory Medicine and Clinical Laboratory Center, Gunma University School of Medicine, Maebashi, Japan.

出版信息

J Lab Clin Med. 1998 Jul;132(1):25-31. doi: 10.1016/s0022-2143(98)90021-x.

DOI:10.1016/s0022-2143(98)90021-x

PMID:9665368

Abstract

The identification of pituitary antigens recognized by human antipituitary antibodies (APAs) is important in evaluating the pathophysiology of multiendocrine disorders linked to autoimmune factors. However, there is no convenient method for the quantitative analysis of circulating APAs. This study reports the development of an enzyme-linked immunosorbent assay (ELISA) for the detection of APAs. APAs were measured by ELISA and confirmed by Western blot analysis in sera from patients with endocrine disorders. APAs were detected frequently in patients with autoimmune thyroiditis, insulin-dependent diabetes mellitus (IDDM), or pituitary dwarfism. Circulating APAs were detected in 18% of patients with autoimmune thyroiditis. Confirmation by Western blot revealed positivity for APAs in the serum of 36% of patients with Hashimoto disease and in 29% of patients with Graves disease. Notably, 39% of patients with IDDM were also positive for APAs by ELISA. The identification of APAs by ELISA may be useful in evaluating autoimmune mechanisms involved in patients with multiendocrine disorders.

摘要

识别被人类抗垂体抗体（APAs）识别的垂体抗原，对于评估与自身免疫因素相关的多内分泌疾病的病理生理学具有重要意义。然而，目前尚无用于循环中APAs定量分析的便捷方法。本研究报告了一种用于检测APAs的酶联免疫吸附测定（ELISA）方法的开发。通过ELISA测定APAs，并通过蛋白质印迹分析对内分泌疾病患者血清进行了验证。在自身免疫性甲状腺炎、胰岛素依赖型糖尿病（IDDM）或垂体性侏儒症患者中经常检测到APAs。在18%的自身免疫性甲状腺炎患者中检测到循环APAs。蛋白质印迹法证实，36%的桥本氏病患者和29%的格雷夫斯病患者血清中的APAs呈阳性。值得注意的是，39%的IDDM患者通过ELISA检测APAs也呈阳性。通过ELISA识别APAs可能有助于评估多内分泌疾病患者的自身免疫机制。