用人抗垂体抗体识别的大鼠垂体抗原的蛋白质免疫印迹分析。

Western blot analysis of rat pituitary antigens recognized by human antipituitary antibodies.

作者信息

Yabe S, Murakami M, Maruyama K, Miwa H, Fukumura Y, Ishii S, Sugiura M, Kobayashi I

机构信息

Department of Laboratory Medicine, Gunma University, School of Medicine, Maebashi, Japan.

出版信息

Endocr J. 1995 Feb;42(1):115-9. doi: 10.1507/endocrj.42.115.

DOI:10.1507/endocrj.42.115

PMID:7599692

Abstract

Antipituitary antibodies have been reported to exist in sera of patients with autoimmune endocrine disorders. In order to investigate the pituitary antigens recognized by antipituitary antibodies, we studied the autoantigens in rat pituitary membrane and cytosolic fractions recognized by human antipituitary antibodies and anti-human pituitary hormone antibodies. Sera from 6 patients which showed positive antipituitary antibodies by immunofluorescence methods were studied. Each serum identified some proteins with 14.5, 22, 47, 49, 65, 84 and 97.5 kDa. Anti-GH antibodies and anti-PRL antibodies identified a positive band with 22 kDa, suggesting that anti-GH and anti-PRL antibodies may be present in patients' sera. These results indicate that Western blot analysis of rat pituitary antigens recognized by human antipituitary antibodies is a useful method to elucidate the pathophysiology of autoimmune endocrine disorders.

摘要

据报道，自身免疫性内分泌疾病患者的血清中存在抗垂体抗体。为了研究抗垂体抗体识别的垂体抗原，我们研究了人抗垂体抗体和抗人垂体激素抗体识别的大鼠垂体膜和胞质组分中的自身抗原。研究了6例通过免疫荧光法显示抗垂体抗体呈阳性的患者的血清。每份血清都鉴定出了一些分子量为14.5、22、47、49、65、84和97.5 kDa的蛋白质。抗生长激素（GH）抗体和抗催乳素（PRL）抗体鉴定出一条22 kDa的阳性条带，表明患者血清中可能存在抗GH和抗PRL抗体。这些结果表明，对人抗垂体抗体识别的大鼠垂体抗原进行蛋白质印迹分析是阐明自身免疫性内分泌疾病病理生理学的一种有用方法。