Paus R, van der Veen C, Eichmüller S, Kopp T, Hagen E, Müller-Röver S, Hofmann U
Department of Dermatology, Charité, Humboldt University, Berlin, Germany.
J Invest Dermatol. 1998 Jul;111(1):7-18. doi: 10.1046/j.1523-1747.1998.00243.x.
In this immunohistomorphometric study, we have defined basic characteristics of the hair follicle (HF) immune system during follicle morphogenesis and cycling in C57BL/6 mice, in relation to the skin immune system. Langerhans cells and gammadelta T cell receptor immunoreactive lymphocytes were the predominant intraepithelial hematopoietic cells in neonatal mouse skin. After their numeric increase in the epidermis, these cells migrated into the HF, although only when follicle morphogenesis was almost completed. In contrast to Langerhans cells, gammadelta T cell receptor immunoreactive lymphocytes entered the HF only via the epidermis. Throughout HF morphogenesis and cycling, both cell types remained strikingly restricted to the distal outer root sheath. On extremely rare occasions, CD4+ or CD8+ alphabetaTC were detected within the HF epithelium or the sebaceous gland. Major histocompatibility complex class II+, MAC-1+ cells of macrophage phenotype and numerous mast cells appeared very early on during HF development in the perifollicular dermis, and the percentage of degranulated mast cells significantly increased during the initiation of synchronized HF cycling (first catagen). During both depilation- and cyclosporine A-induced HF cycling, the numbers of intrafollicular Langerhans cells, gammadelta T cell receptor immunoreactive lymphocytes, and perifollicular dermal macrophages fluctuated significantly. Yet, no numeric increase of perifollicular macrophages was detectable during HF regression, questioning their proposed role in catagen induction. In summary, the HF immune system is generated fairly late during follicle development, shows striking differences to the extrafollicular skin immune system, and undergoes substantial hair cycle-associated remodeling. In addition, synchronized HF cycling is accompanied by profound alterations of the skin immune system.
在这项免疫组织形态计量学研究中，我们确定了C57BL/6小鼠毛囊形态发生和周期性变化过程中毛囊（HF）免疫系统的基本特征，并将其与皮肤免疫系统进行了关联研究。朗格汉斯细胞和γδT细胞受体免疫反应性淋巴细胞是新生小鼠皮肤中主要的上皮内造血细胞。在表皮中数量增加后，这些细胞迁移到毛囊中，不过仅在毛囊形态发生几乎完成时才会如此。与朗格汉斯细胞不同，γδT细胞受体免疫反应性淋巴细胞仅通过表皮进入毛囊。在整个毛囊形态发生和周期性变化过程中，这两种细胞类型都显著局限于远端外根鞘。在极罕见的情况下，在毛囊上皮或皮脂腺内可检测到CD4 +或CD8 +αβTC。主要组织相容性复合体II类阳性、具有巨噬细胞表型的MAC - 1阳性细胞以及大量肥大细胞在毛囊发育早期就在毛囊周围真皮中出现，并且在同步毛囊周期性变化开始（第一次退行期）时，脱颗粒肥大细胞的百分比显著增加。在脱毛诱导和环孢素A诱导的毛囊周期性变化过程中，毛囊内朗格汉斯细胞、γδT细胞受体免疫反应性淋巴细胞以及毛囊周围真皮巨噬细胞的数量均有显著波动。然而，在毛囊退化过程中未检测到毛囊周围巨噬细胞数量增加，这对其在退行期诱导中所提出的作用提出了质疑。总之，毛囊免疫系统在毛囊发育过程中产生得相当晚，与毛囊外皮肤免疫系统存在显著差异，并且经历了与毛发周期相关的大量重塑。此外，同步毛囊周期性变化伴随着皮肤免疫系统的深刻改变。
