Moffatt J D, Dumsday B, McLean J R
Department of Zoology, The University of Melbourne, Parkville, Victoria, Australia.
Neurosci Lett. 1998 May 22;248(1):37-40. doi: 10.1016/s0304-3940(98)00321-8.
The possibility that non-adrenergic, non-cholinergic (NANC) neurons innervating the guinea-pig trachea may be located within the oesophagus has been investigated using an in vitro retrograde tracing technique. The cervical trachea and oesophagus were excised from guinea-pigs and Dil was applied to a 5 mm region of the trachealis muscle. These preparations were maintained in organotypic culture for 3 days and processed for immunohistochemistry. A mean of 44 (4 neural cell bodies in the oesophageal myenteric plexus were found to be labelled by Dil. The vast majority of these neurons contained nitric oxide synthase, vasoactive intestinal polypeptide and neuropeptide Y. It is suggested that the population of neurons identified in this study are postganglionic parasympathetic neurons mediating NANC relaxation of the trachealis muscle in this species.
利用体外逆行追踪技术，研究了支配豚鼠气管的非肾上腺素能、非胆碱能（NANC）神经元是否可能位于食管内。从豚鼠身上切除颈段气管和食管，并将碘化丙啶（Dil）应用于气管肌5毫米区域。这些标本在器官型培养中维持3天，然后进行免疫组织化学处理。平均有44个（食管肌间神经丛中的4个神经细胞体）被Dil标记。这些神经元绝大多数含有一氧化氮合酶、血管活性肠肽和神经肽Y。研究表明，本研究中鉴定出的神经元群体是该物种中介导气管肌NANC舒张的节后副交感神经元。
