牙龈卟啉单胞菌的分离及龈沟液中菌毛抗原特异性免疫球蛋白A的检测
Isolation of Porphyromonas gingivalis and detection of immunoglobulin A specific to fimbrial antigen in gingival crevicular fluid.
作者信息
Condorelli F, Scalia G, Calì G, Rossetti B, Nicoletti G, Lo Bue A M
机构信息
Institute of Microbiology, University of Catania, Italy.
出版信息
J Clin Microbiol. 1998 Aug;36(8):2322-5. doi: 10.1128/JCM.36.8.2322-2325.1998.
The present study evaluated the prevalence of Porphyromonas gingivalis and the correlation between the bacterial culture method and the detection of immunoglobulin A (IgA) specific to the P. gingivalis fimbrial antigen in gingival crevicular fluid (GCF). P. gingivalis was isolated from 78.3% of subgingival plaque samples obtained from active sites and 34.7% of those from inactive sites of periodontal patients. P. gingivalis was isolated from only 4.7% of healthy subjects (control group). Immunoglobulins specific to the P. gingivalis fimbrial antigen were detected by enzyme-linked immunosorbent assay (ELISA). The overall agreement between the results of the P. gingivalis culture method and the results of specific IgA detection in periodontal patients was 71.7% for active sites and 58.7% for inactive sites. IgA specific to P. gingivalis was absent in GCF from all of the sites of healthy subjects. The results suggest that P. gingivalis is associated with the local production of specific IgA. The detection of IgA antibodies specific to P. gingivalis in GCF by ELISA may be used as a predictive parameter to reveal the early phase of the activation of recurrent periodontal infections.
本研究评估了牙龈卟啉单胞菌的患病率，以及细菌培养方法与龈沟液（GCF）中牙龈卟啉单胞菌菌毛抗原特异性免疫球蛋白A（IgA）检测之间的相关性。从牙周炎患者活动部位获取的龈下菌斑样本中，78.3%分离出牙龈卟啉单胞菌；从不活动部位获取的样本中，34.7%分离出该菌。仅4.7%的健康受试者（对照组）分离出牙龈卟啉单胞菌。采用酶联免疫吸附测定（ELISA）检测牙龈卟啉单胞菌菌毛抗原特异性免疫球蛋白。牙周炎患者活动部位牙龈卟啉单胞菌培养结果与特异性IgA检测结果的总体一致性为71.7%，不活动部位为58.7%。健康受试者所有部位的龈沟液中均未检测到牙龈卟啉单胞菌特异性IgA。结果表明，牙龈卟啉单胞菌与特异性IgA的局部产生有关。通过ELISA检测龈沟液中牙龈卟啉单胞菌特异性IgA抗体，可作为揭示复发性牙周感染激活早期阶段的预测参数。
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