Wada Y, Igawa M, Shiina H, Shigeno K, Yokogi H, Urakami S, Yoneda T, Maruyama R
Department of Urology, Shimane Medical University, Izumo, Japan.
Br J Cancer. 1998 Jun;77(11):2003-7. doi: 10.1038/bjc.1998.332.
To evaluate the significance of chromosomal aberrations in renal cell carcinoma, fluorescence in situ hybridization (FISH) was used to determine its prevalence and correlation with clinical parameters of malignancy. In addition, correlation of chromosomal aberration with Ki 67 expression was analysed. We performed FISH with chromosome-specific DNA probes, and the signal number of pericentromeric sequences on chromosomes 3, 7, 9 and 17 was detected within interphase nuclei in touch preparations from tumour specimen. The incidence of loss of chromosome 3 was significantly higher than those of chromosomes 7, 9 and 17 (P < 0.001, P = 0.03 and P < 0.001 respectively). Hyperdiploid aberration of chromosomes 3 and 17 was significantly correlated with tumour stage (P = 0.03, P = 0.02 respectively), whereas hyperdiploid aberration of chromosome 9 was associated with nuclear grade (P = 0.04). Disomy of chromosome 7 was correlated with venous involvement (P = 0.04). Ki 67 expression was significantly associated with hyperdiploid aberration of chromosome 17 (P = 0.01), but not with aberration of chromosome 3. There was a significant relationship between hyperdiploid aberration of chromosome 7 and Ki 67 expression (P = 0.01). In conclusions, gain of chromosome 17 may reflect tumour development, and aberration of chromosome 7 may affect metastatic potential of malignancy, whereas loss of chromosome 3 may be associated with early stage of tumour development in renal cell carcinoma.
为评估染色体畸变在肾细胞癌中的意义，采用荧光原位杂交（FISH）技术来确定其发生率以及与恶性肿瘤临床参数的相关性。此外，还分析了染色体畸变与Ki 67表达的相关性。我们使用染色体特异性DNA探针进行FISH检测，并在肿瘤标本的触片间期核内检测3号、7号、9号和17号染色体着丝粒周围序列的信号数量。3号染色体缺失的发生率显著高于7号、9号和17号染色体（分别为P < 0.001、P = 0.03和P < 0.001）。3号和17号染色体的超二倍体畸变与肿瘤分期显著相关（分别为P = 0.03、P = 0.02），而9号染色体的超二倍体畸变与核分级相关（P = 0.04）。7号染色体二体与静脉侵犯相关（P = 0.04）。Ki 67表达与17号染色体的超二倍体畸变显著相关（P = 0.01），但与3号染色体畸变无关。7号染色体的超二倍体畸变与Ki 67表达之间存在显著关系（P = 0.01）。总之，17号染色体增加可能反映肿瘤进展，7号染色体畸变可能影响恶性肿瘤的转移潜能，而3号染色体缺失可能与肾细胞癌肿瘤发展的早期阶段相关。
