Yokogi H, Wada Y, Moriyama-Gonda N, Igawa M, Ishibe T
Department of Urology, Shimane Medical University, Izumo, Japan.
Br J Urol. 1996 Nov;78(5):699-703. doi: 10.1046/j.1464-410x.1996.01786.x.
To investigate the relationship between genomic heterogeneity and tumour grade, stage and DNA content in 30 transitional cell carcinomas (TCCs) of the urinary bladder.
Tissue specimens from 30 patients (25 men and five women) with newly diagnosed TCC of the urinary bladder were examined for genomic heterogeneity using fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes; the copy number of pericentromeric sequences on chromosomes 7, 9 and 17 was detected within interphase nuclei in contact preparations from the tumour specimens.
The aneusomy of chromosomes 7, 9 and 17 was significantly higher in aneuploid than in diploid tumours (P < 0.001). Tumour grade and stage were strongly associated with aneusomy for chromosome 17 (P < 0.01, P < 0.001, respectively). The aneusomy of chromosomes 7 and 9 were significantly correlated with increasing tumour stage (P < 0.001), but not with tumour grade.
These results suggest that the measurement of aneusomy using FISH, especially for chromosome 17, in bladder cancer may offer a new objective and quantitative assay of the biological potential of individual tumours.
研究30例膀胱移行细胞癌（TCC）的基因组异质性与肿瘤分级、分期及DNA含量之间的关系。
采用染色体特异性DNA探针的荧光原位杂交（FISH）技术，对30例新诊断为膀胱TCC的患者（25例男性和5例女性）的组织标本进行基因组异质性检测；在肿瘤标本的接触制片的间期核内检测7号、9号和17号染色体着丝粒周围序列的拷贝数。
非整倍体肿瘤中7号、9号和17号染色体的非整倍体率显著高于二倍体肿瘤（P < 0.001）。肿瘤分级和分期与17号染色体的非整倍体密切相关（分别为P < 0.01，P < 0.001）。7号和9号染色体的非整倍体与肿瘤分期增加显著相关（P < 0.001），但与肿瘤分级无关。
这些结果表明，利用FISH检测非整倍体，尤其是17号染色体，可能为膀胱癌个体肿瘤的生物学潜能提供一种新的客观定量检测方法。
