Savitsky P A, Rojkova A M, Tishkov V I, Ouporov I V, Rudenskaya G N, Gazaryan I G
Department of Chemical Enzymology, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia.
Biochemistry (Mosc). 1998 Jun;63(6):629-33.
Application of computer methods allowed us to demonstrate that plant peroxidases and auxin-binding proteins contain structurally similar fragments. The mapping of the fragments was done using a model structure of horseradish peroxidase. Five of six structurally similar fragments belong to the distal domain and form a subdomain in plant peroxidases that includes the distal heme-coordinating sequence, LHFHDC (amino acid residues 39-44 in horseradish peroxidase). The existence of a substrate-binding site for indole-3-acetic acid in the distal subdomain comprising helices A (whole), B (middle), C (beginning), and D (whole) and the loop between helices D and D' is discussed.
计算机方法的应用使我们能够证明植物过氧化物酶和生长素结合蛋白含有结构相似的片段。这些片段的定位是利用辣根过氧化物酶的模型结构完成的。六个结构相似的片段中有五个属于远端结构域，在植物过氧化物酶中形成一个亚结构域，该亚结构域包括远端血红素配位序列LHFHDC（辣根过氧化物酶中的氨基酸残基39 - 44）。讨论了在由螺旋A（整个）、B（中间）、C（起始）和D（整个）以及螺旋D和D'之间的环组成的远端亚结构域中存在吲哚 - 3 - 乙酸的底物结合位点。
