Blahová J, Hupková-Lesická M, Králiková K, Krcméry V, Kubonová K, Torsová V, Bartoníková N, Schäfer V
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
J Chemother. 1998 Jun;10(3):215-20. doi: 10.1179/joc.1998.10.3.215.
This paper describes transferability of antibiotic resistance determinants in clinical isolates of Pseudomonas aeruginosa resistant to imipenem, cefotaxime and ceftazidime obtained from different clinical settings in three different countries. Two strains of Enterobacteriaceae (Escherichia coli K-12 and Proteus mirabilis P-38) and two strains of P. aeruginosa (PAO and ML) were used as recipient strains. The conjugative transfer of resistance was very specific, i.e. donor strains of P. aeruginosa transferred individual resistance determinants either to recipient strains E. coli K-12 and P. mirabilis P-38, or to P. aeruginosa PAO or ML. In a case when three different species (P. aeruginosa, Acinetobacter calcoaceticus, Stenotrophomonas maltophilia) were isolated from a single patient, a block of resistance determinants was transferred both to Enterobacteriaceae and P. aeruginosa recipients. A single plasmid with rather specific properties was suspected of being exchanged among strains of these different species. It was recommended to study the overall situation of transferability of individual resistance determinants in bacterial strains belonging to various species of nosocomial bacteria, especially of P. aeruginosa, by using a rather specific methodology.
本文描述了从三个不同国家的不同临床环境中分离出的对亚胺培南、头孢噻肟和头孢他啶耐药的铜绿假单胞菌临床分离株中抗生素耐药决定因子的转移性。使用两株肠杆菌科细菌（大肠杆菌K-12和奇异变形杆菌P-38）以及两株铜绿假单胞菌（PAO和ML）作为受体菌株。耐药性的接合转移非常具有特异性，即铜绿假单胞菌供体菌株将单个耐药决定因子转移至受体菌株大肠杆菌K-12和奇异变形杆菌P-38，或转移至铜绿假单胞菌PAO或ML。在从一名患者中分离出三种不同菌种（铜绿假单胞菌、醋酸钙不动杆菌、嗜麦芽窄食单胞菌）的情况下，一组耐药决定因子被转移至肠杆菌科细菌和铜绿假单胞菌受体。怀疑一种具有相当特殊性质的单一质粒在这些不同菌种的菌株之间进行交换。建议通过使用一种相当特殊的方法来研究属于各种医院细菌菌种，尤其是铜绿假单胞菌的细菌菌株中单个耐药决定因子转移性的总体情况。
