Olson B A, Ali S M, Contino L C, Brooks D P, Laping N J
Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19416, USA.
Pharmacology. 1998 Jul;57(1):13-9. doi: 10.1159/000028221.
The effect of the angiotensin-converting enzyme inhibitor captopril on clusterin mRNA was examined in partially nephrectomized male rats. Urine protein excretion was measured 3, 7, and 28 days after removal of five sixths of the renal mass. Nephrectomy caused a progressive increase in clusterin mRNA levels in the remnant kidney. Maximal clusterin mRNA levels occurred 7 days after nephrectomy and declined 28 days after nephrectomy. Captopril, 250 mg/ml in drinking water, prevented the injury-induced increase in clusterin mRNA at 7 and 28 days. Captopril had no effect on clusterin in sham-operated rats. As expected, the urine protein excretion increased progressively after nephrectomy, and this was attenuated by administration of captopril in the drinking water. Therefore, clusterin is a marker of renal injury which, along with proteinuria, is modulated by angiotensin-converting enzyme inhibition.
在部分肾切除的雄性大鼠中检测了血管紧张素转换酶抑制剂卡托普利对簇集素mRNA的影响。在切除六分之五的肾脏组织后3天、7天和28天测量尿蛋白排泄量。肾切除导致残余肾脏中簇集素mRNA水平逐渐升高。簇集素mRNA水平在肾切除后7天达到最高，在肾切除后28天下降。饮用水中浓度为250 mg/ml的卡托普利可在7天和28天时阻止损伤诱导的簇集素mRNA增加。卡托普利对假手术大鼠的簇集素没有影响。正如预期的那样，肾切除术后尿蛋白排泄量逐渐增加，而饮用水中给予卡托普利可使其减轻。因此，簇集素是肾损伤的一个标志物，它与蛋白尿一起，可通过血管紧张素转换酶抑制作用来调节。
