Jung-Ha H, Kim D, Lee S B, Hong S I, Park S Y, Huh J, Kim C W, Kim S S, Lee Y, Choi S S, Shin H S
Department of Biology, Andong National University, Korea.
Hum Pathol. 1998 Jul;29(7):723-8. doi: 10.1016/s0046-8177(98)90282-9.
Bfl-1, a member of the Bcl-2 gene family, blocks p53-mediated apoptosis and has oncogenic transforming activity. In normal tissues, the transcript of Bfl-1 is expressed abundantly in bone marrow and at a low level in several other tissues. In previous experiments, elevated expression of Bfl-1 was observed by Northern analysis of stomach cancer samples. To study the role of Bfl-1 in normal cell development and in tumorigenesis, we have analyzed the expression of Bfl-1 in normal and tumor tissues by the in situ hybridization technique. The Bfl-1 transcript was detected in the white pulp of the spleen and in the germinal center of lymphatic tissues. In tumor tissues, its expression was preferentially detected in infiltrating inflammatory cells rather than in cancer cells, suggesting that Bfl-1 is not involved in tumorigenesis.
Bfl-1是Bcl-2基因家族的成员之一，可阻断p53介导的细胞凋亡并具有致癌转化活性。在正常组织中，Bfl-1的转录本在骨髓中大量表达，而在其他几种组织中表达水平较低。在先前的实验中，通过对胃癌样本的Northern分析观察到Bfl-1表达升高。为了研究Bfl-1在正常细胞发育和肿瘤发生中的作用，我们采用原位杂交技术分析了Bfl-1在正常组织和肿瘤组织中的表达。在脾脏白髓和淋巴组织生发中心检测到Bfl-1转录本。在肿瘤组织中，其表达优先在浸润性炎症细胞中检测到，而不是在癌细胞中，这表明Bfl-1不参与肿瘤发生。
