Takemoto Y, Furuta M, Sato M, Findell P R, Ramble W, Hashimoto Y
Institute of Immunology, Syntex-Roche, Chiba, Japan.
J Immunol. 1998 Jul 15;161(2):625-30.
To analyze the growth factor receptor-bound protein 2 (Grb2) signaling pathway in lymphoid cells, we used expression cloning to isolate the genes encoding proteins that associate with Grb2. We find that the Src homology 3 domains of Grb2 directly associate, in vitro and in vivo, with murine hemopoietic specific protein 1 (HS1), a protein identical to Lck-binding protein 1. Because HS1 associates with the p56(lck) and p59(lyn) tyrosine kinases in vitro and in vivo, and becomes tyrosine phosphorylated upon various receptor stimulations, our present data suggest that HS1 mediates linkage between Lck or Lyn and Grb2 in lymphoid lineage cells.
为了分析淋巴细胞中生长因子受体结合蛋白2（Grb2）信号通路，我们利用表达克隆技术分离出编码与Grb2相关蛋白的基因。我们发现，Grb2的Src同源3结构域在体外和体内均直接与小鼠造血特异性蛋白1（HS1）相关联，HS1与Lck结合蛋白1相同。由于HS1在体外和体内均与p56(lck)和p59(lyn)酪氨酸激酶相关联，并在各种受体刺激下发生酪氨酸磷酸化，我们目前的数据表明，HS1在淋巴谱系细胞中介导Lck或Lyn与Grb2之间的联系。
