表达甜菜坏死黄脉病毒（BNYVV）外壳蛋白特异性单链抗体片段（scFv）的本氏烟草植株在感染早期对该病毒的侵染以及在感染后期对其致病作用具有部分抗性。

Nicotiana benthamiana plants expressing beet necrotic yellow vein virus (BNYVV) coat protein-specific scFv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection.

作者信息

Fecker L F, Koenig R, Obermeier C

机构信息

Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.

出版信息

Arch Virol. 1997;142(9):1857-63. doi: 10.1007/s007050050203.

DOI:10.1007/s007050050203

PMID:9672643

Abstract

Transgenic plants of Nicotiana benthamiana expressing single chain antibody fragments (scFv) specific for the coat protein of beet necrotic yellow vein virus (BNYVV) and non-expressing control plants were inoculated with BNYVV mechanically and by means of the vector Polymyxa betae. The scFv were presumably expressed in the endoplasmic reticulum (ER). The average time needed for infections to become detectable was longer in the scFv-expressing plants than in the non-expressing control plants. In addition, the scFv-expressing plants were partially protected against the pathogenic effects exerted by the virus on N. benthamiana plants in the late stages of infection.

摘要

用甜菜坏死黄脉病毒（BNYVV）外壳蛋白特异性单链抗体片段（scFv）表达的本氏烟草转基因植株和非表达对照植株，通过机械接种和借助载体甜菜多黏菌接种BNYVV。scFv可能在内质网（ER）中表达。在scFv表达植株中感染变得可检测所需的平均时间比非表达对照植株更长。此外，在感染后期，scFv表达植株受到部分保护，免受病毒对本氏烟草植株施加的致病作用。