State Key Laboratory for Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
Laboratory of Phytopathology, College of Chemistry Biology and Environment, Yuxi Normal University, Yuxi 653100, China.
Viruses. 2020 Mar 12;12(3):310. doi: 10.3390/v12030310.
(BNYVV) infections induce stunting and leaf curling, as well as root and floral developmental defects and leaf senescence in . A microarray analysis with probes capable of detecting 1596 candidate microRNAs (miRNAs) was conducted to investigate differentially expressed miRNAs and their targets upon BNYVV infection of plants. Eight species-specific miRNAs of were identified. Comprehensive characterization of the microRNA profile in response to the BNYVV infection revealed that 129 miRNAs were altered, including four species-specific miRNAs. The targets of the differentially expressed miRNAs were predicted accordingly. The expressions of miR164, 160, and 393 were up-regulated by BNYVV infection, and those of their target genes, , , and , were down-regulated. , which is a target of miR396, was also down-regulated. Further genetic analysis of by -induced gene silencing, assay confirmed the involvement of in the symptom development during BNYVV infection. BNYVV infection also induced the up-regulation of miR168 and miR398. The miR398 was predicted to target , and silencing of could enhance plant resistance against viruses, suggesting the activation of primary defense response to BNYVV infection in . These results provide a global profile of miRNA changes induced by BNYVV infection and enhance our understanding of the mechanisms underlying BNYVV pathogenesis.
（BNYVV）感染会导致植物生长迟缓、叶片卷曲，以及根和花的发育缺陷和叶片衰老。利用能够检测 1596 个候选 microRNAs（miRNAs）的微阵列分析，研究了 BNYVV 感染 植物后差异表达的 miRNAs 及其靶标。鉴定出了 8 种特异于 的 miRNAs。全面表征 对 BNYVV 感染的 microRNA 谱表明，有 129 个 miRNAs 发生了改变，包括 4 种特异于 的 miRNAs。相应地预测了差异表达 miRNAs 的靶标。miR164、160 和 393 的表达受 BNYVV 感染上调，其靶基因 、 、 和 的表达下调。miR396 的靶标 也下调。通过 -诱导的基因沉默对 进行进一步的遗传分析，验证了 参与 BNYVV 感染期间症状的发展。BNYVV 感染还诱导了 miR168 和 miR398 的上调。miR398 被预测靶向 ，沉默 可以增强植物对病毒的抗性，表明在 中对 BNYVV 感染的初级防御反应被激活。这些结果提供了 BNYVV 感染诱导的 miRNA 变化的全局图谱，并增强了我们对 BNYVV 发病机制的理解。
