用于在疫苗菌株流产布鲁氏菌S19中表达外源蛋白的载体开发。
Vector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19.
作者信息
Comerci D J, Pollevick G D, Vigliocco A M, Frasch A C, Ugalde R A
机构信息
Instituto de Investigaciones Biotecnologicas, Universidad Nacional de General San Martin, INTI, San Martin, Provincia de Buenos Aires, Argentina.
出版信息
Infect Immun. 1998 Aug;66(8):3862-6. doi: 10.1128/IAI.66.8.3862-3866.1998.
Abstract
A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid pBEV. As a reporter protein, a repetitive antigen of Trypanosoma cruzi was used. The recombinant fusion protein is stably expressed and secreted into the Brucella periplasmic space, inducing a good antibody response against the T. cruzi antigen. The expression of the repetitive antigen in Brucella neither altered its growth pattern nor generated a toxic or lethal effect during experimental infection. The application of this strategy for the generation of live recombinant vaccines and the tagging of B. abortus S19 vaccine is discussed. This is the first time that a recombinant protein has been expressed in the periplasm of brucellae.
摘要
通过使用包含布鲁氏菌bcsp31基因调控序列和信号肽的DNA片段，构建了一种用于在疫苗株布鲁氏菌流产菌S19中表达外源抗原的载体。该片段克隆于广宿主范围质粒pBBR4MCS中，得到质粒pBEV。使用克氏锥虫的一种重复抗原作为报告蛋白。重组融合蛋白在布鲁氏菌周质空间中稳定表达并分泌，诱导产生针对克氏锥虫抗原的良好抗体反应。克氏锥虫重复抗原在布鲁氏菌中的表达既未改变其生长模式，在实验感染期间也未产生毒性或致死效应。讨论了该策略在生产活重组疫苗和布鲁氏菌流产菌S19疫苗标记方面的应用。这是首次在布鲁氏菌周质中表达重组蛋白。
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本文引用的文献
1
The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain.克氏锥虫转唾液酸酶的重复结构域增强了针对催化结构域的免疫反应。
J Infect Dis. 1998 Feb;177(2):431-6. doi: 10.1086/514199.
2
T cell-independent response to Brucella-insulin identifies a preimmune repertoire for insulin.对布鲁氏菌-胰岛素的非T细胞依赖性反应确定了胰岛素的免疫前库。
J Immunol. 1997 Sep 1;159(5):2334-41.
3
Brucellosis: an overview.布鲁氏菌病概述
Emerg Infect Dis. 1997 Apr-Jun;3(2):213-21. doi: 10.3201/eid0302.970219.
4
5
Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis.流产布鲁氏菌基因的克隆及表达的26千道尔顿周质蛋白的特性分析：诊断的潜在用途
J Clin Microbiol. 1996 Jan;34(1):165-9. doi: 10.1128/jcm.34.1.165-169.1996.
6
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes.四种新型的广宿主范围克隆载体pBBR1MCS的衍生物，携带不同的抗生素抗性盒。
Gene. 1995 Dec 1;166(1):175-6. doi: 10.1016/0378-1119(95)00584-1.
7
Periplasmic location of Brucella abortus Cu/Zn superoxide dismutase.流产布鲁氏菌铜/锌超氧化物歧化酶的周质定位
Vet Microbiol. 1994 Feb;38(4):307-14. doi: 10.1016/0378-1135(94)90149-x.
8
pBBR1MCS: a broad-host-range cloning vector.pBBR1MCS：一种广宿主范围的克隆载体。
Biotechniques. 1994 May;16(5):800-2.
9
10
In vivo and in vitro stability of the broad-host-range cloning vector pBBR1MCS in six Brucella species.广泛宿主范围克隆载体pBBR1MCS在六种布鲁氏菌中的体内和体外稳定性
Plasmid. 1995 Jan;33(1):51-7. doi: 10.1006/plas.1995.1006.
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